Coxsackievirus A16 induced neurological disorders in young gerbils which could serve as a new animal model for vaccine evaluation.
Coxsackievirus A16 induced neurological disorders in young gerbils which could serve as a new animal model for vaccine evaluation.
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DOI：
10.1038/srep34299
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发表时间：
2016-09-26
影响因子：
4.6
通讯作者：
Zhu HP
中科院分区：
文献类型：
--
作者：
Sun YS;Li YJ;Xia Y;Xu F;Wang WW;Yang ZN;Lu HJ;Chen ZP;Miao ZP;Liang WF;Xu ZY;Dong HJ;Qiu DH;Zhu ZY;van der Veen S;Qian J;Zhou B;Yao PP;Zhu HP
柯萨奇病毒A16型（Coxsackievirus A16，CA16）是亚太地区与人类手足口病（HFMD）相关的主要病原体之一。虽然CA16感染通常是轻微的，但有严重的神经系统表现甚至死亡的报道。CA16发病机制和疫苗开发的研究受到严重阻碍，因为目前可用的小动物模型显示出很大的局限性。在这项研究中，沙鼠（长爪沙鼠）的适用性进行了调查，作为动物模型，研究CA 16的发病机制和疫苗的发展。我们的研究结果表明，沙鼠的年龄达21天是完全敏感的CA 16和所有在感染后5天内死亡。CA16对沙鼠的骨骼肌、脊髓和脑干有趋化性，并可引起严重的病变，包括坏死。此外，给予沙鼠的灭活CA 16全病毒疫苗能够为沙鼠提供针对致死剂量的CA 16毒株的完全保护。这些结果表明，沙鼠是研究CA 16感染和疫苗开发的合适动物模型。
Coxsackievirus A16 (CA16) is one of the major pathogens associated with human hand, foot, and mouth disease (HFMD) in the Asia-pacific region. Although CA16 infections are generally mild, severe neurological manifestations or even death has been reported. Studies on CA16 pathogenesis and vaccine development are severely hampered because the small animal models that are currently available show major limitations. In this study, gerbils (Meriones unguiculatus) were investigated for their suitability as an animal model to study CA16 pathogenesis and vaccine development. Our results showed that gerbils up to the age of 21 days were fully susceptible to CA16 and all died within five days post-infection. CA16 showed a tropism towards the skeletal muscle, spinal cord and brainstem of gerbils, and severe lesions, including necrosis, were observed. In addition, an inactivated CA16 whole-virus vaccine administrated to gerbils was able to provide full protection to the gerbils against lethal doses of CA16 strains. These results demonstrate that gerbils are a suitable animal model to study CA16 infection and vaccine development.
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