Capillary electrochromatography of peptides on a column packed with tentacular weak cation-exchanger particles.
Capillary electrochromatography of peptides on a column packed with tentacular weak cation-exchanger particles.
复制标题
在装有触手弱阳离子交换剂颗粒的柱上对肽进行毛细管电色谱分析。
DOI：
10.1016/s0021-9673(02)00070-5
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Horváth,Csaba
中科院分区：
文献类型：
--
作者：
Zhang,Jian;Zhang,Shuhong;Horváth,Csaba
制备了一种具有触角状结构的硅基弱阳离子交换剂颗粒，用于毛细管电色谱（CEC）分离带正电荷的样品组分。首先用甲基丙烯酸3-（三甲氧基甲硅烷基）丙酯硅烷化二氧化硅珠，甲基丙烯酸3-（三甲氧基甲硅烷基）丙酯用作异双官能连接体，其在第二步中通过在水溶液中的自由基聚合与2-丙烯酰胺基乙醇酸反应。在填充毛细管柱上，以NaCl为移动的相调节剂，采用等度洗脱CEC，基本上实现了碱性肽的基线分离。通过考察盐浓度对肽迁移行为的影响，研究了电色谱过程中的保留机理。在假定带电迁移体的总迁移速率为色谱洗脱速率和电泳迁移速率之和的基础上，估算了带电样品组分在电色谱过程中的色谱保留因子k′ lc。根据实验结果估算的k′ lc值与摩尔盐浓度以双对数标度作图。线性相关性与传统离子交换色谱理论预测值吻合较好。CEC结果的比较，获得与开放的管状和填充的毛细管柱具有相同的保留功能的固定相，支持的概念，在列中的相比的变化提供了一个额外的手段来调制的电色谱迁移行为。
Silica-based, tentacular weak cation-exchanger particles were prepared for use as the stationary phase in the separation of positively charged sample components by capillary electrochromatography (CEC). Silica beads were first silanized with 3-(trimethoxysilyl) propyl methacrylate that served as a heterobifunctional linker, which reacted with 2-acrylamidoglycolic acid in a second step by radical polymerization in aqueous solution. Baseline separation of basic peptides with good column efficiency was obtained on packed capillary columns by isocratic elution CEC with NaCl as the mobile phase modulator. The retention mechanism in the electrochromatographic process was studied by examining the effect of salt concentration on the migration behavior of the peptides. The chromatographic retention factor k′lcfor charged sample components in the electrochromatographic process was estimated on the assumption that the overall migration rate of a charged migrant can be taken as the sum of the rate of chromatographic elution and the rate of electrophoretic migration. The estimated k′lcvalues from experimental results were plotted against the molal salt concentration on a double logarithmic scale. The linear correlation is in good agreement with the prediction by the theory on the basis of traditional ion-exchange chromatography. The comparison of CEC results, obtained with open tubular and packed capillary columns having the same retentive functions as the stationary phase, supports the notion that variation of the phase ratio in the column offers an additional means to modulate the electrochromatographic migration behavior.
登录
查看更多内容
流动相添加剂对薄膜和全多孔吸附剂反相色谱中肽保留的影响。
DOI：
10.1016/0021-9673(95)01019-x
发表时间：
1996
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Corradini,D;Kalghatgi,K;Horváth,C
通讯作者：
Horváth,C
DOI：
10.1016/s0021-9673(96)00864-3
发表时间：
1997-02
期刊：
Journal of chromatography. A
影响因子：
--
作者：
J. Pesek;M. Matyska;L. Mauskar
通讯作者：
J. Pesek;M. Matyska;L. Mauskar
影响因子：
4.1
作者：
Asiaie, R;Huang, X;Horváth, C
通讯作者：
Horváth, C
通过高效液相色谱、毛细管区带电泳和电动色谱中的虚拟迁移距离确定分离参数。
DOI：
10.1016/0021-9673(96)00306-8
发表时间：
1996
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Rathore,AS;Horváth,C
通讯作者：
Horváth,C
使用阳离子丙烯酸整料对蛋白质和肽进行毛细管电色谱分析。
DOI：
10.1016/s0021-9673(00)00250-8
发表时间：
2000
期刊：
Journal of chromatography. A
影响因子：
--
作者：
Zhang,S;Huang,X;Zhang,J;Horváth,C
通讯作者：
Horváth,C