Rapid detection of novel coronavirus SARS-CoV-2 by RT-LAMP coupled solid-state nanopores.
Rapid detection of novel coronavirus SARS-CoV-2 by RT-LAMP coupled solid-state nanopores.
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DOI：
10.1016/j.bios.2021.113759
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发表时间：
2022-02-01
影响因子：
12.6
通讯作者：
Guan W
中科院分区：
文献类型：
--
作者：
Tang Z;Nouri R;Dong M;Yang J;Greene W;Zhu Y;Yon M;Nair MS;Kuchipudi SV;Guan W
目前由SARS-CoV-2(严重急性呼吸综合征冠状病毒-2)引起的新冠肺炎大流行已经引起了重大的公共卫生问题。为了及早发现和控制疾病传播，迫切需要对SARS-CoV-2进行快速和准确的检测。在这里，我们提出了一种用于SARS-CoV-2快速检测的RT-LAMP耦合玻璃纳米孔数字计数方法。我们验证并比较了两种针对核衣壳(N)和包膜(E)基因的一锅RT-LAMP检测方法。核衣壳试验因其快速阳性和较好的拷贝数敏感性而被采用。对于测试样本的定性阳性/阴性分类，我们使用玻璃纳米孔对RT-LAMP扩增进行数字计数，并以阈值作为事件发生率的基准。由于其固有的单分子敏感性，纳米孔传感器可以更快地捕捉到放大动力学(快速到阳性)。我们使用添加唾液样本和新冠肺炎临床鼻咽拭子样本验证了RT-LAMP耦合玻璃纳米孔数字计数方法检测SARS-CoV-2的有效性。结果表明，所获得的结果与金标准RT-PCR方法具有很好的一致性。凭借其集成能力，电子纳米孔数字计数平台具有巨大的潜力，可以为SARS-CoV-2提供快速、灵敏和特异的护理点检测。
The current pandemic of COVID-19 caused by SARS-CoV-2 (severe acute respiratory syndrome coronavirus-2) has raised significant public health concerns. Rapid and accurate testing of SARS-CoV-2 is urgently needed for early detection and control of the disease spread. Here, we present an RT-LAMP coupled glass nanopore digital counting method for rapid detection of SARS-CoV-2. We validated and compared two one-pot RT-LAMP assays targeting nucleocapsid (N) and envelop (E) genes. The nucleocapsid assay was adopted due to its quick time to positive and better copy number sensitivity. For qualitative positive/negative classification of a testing sample, we used the glass nanopore to digitally count the RT-LAMP amplicons and benchmarked the event rate with a threshold. Due to its intrinsic single molecule sensitivity, nanopore sensors could capture the amplification dynamics more rapidly (quick time to positive). We validated our RT-LAMP coupled glass nanopore digital counting method for SARS-CoV-2 detection by using both spiked saliva samples and COVID-19 clinical nasopharyngeal swab samples. The results obtained showed excellent agreement with the gold standard RT-PCR assay. With its integration capability, the electronic nanopore digital counting platform has significant potential to provide a rapid, sensitive, and specific point-of-care assay for SARS-CoV-2.
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使用大肠杆菌作为模型系统量化环介导等温扩增 (LAMP) 的经验方法。
影响因子：
3.7
作者：
Subramanian S;Gomez RD
通讯作者：
Gomez RD
DOI：
10.1073/pnas.2014739117
发表时间：
2020-09-15
影响因子：
11.1
作者：
Ganguli A;Mostafa A;Berger J;Aydin MY;Sun F;Ramirez SAS;Valera E;Cunningham BT;King WP;Bashir R
通讯作者：
Bashir R
DOI：
10.1146/annurev-anchem-061417-125903
发表时间：
2019-01-01
期刊：
ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, VOL 12
影响因子：
--
作者：
Albrecht, Tim
通讯作者：
Albrecht, Tim
DOI：
10.1016/j.medj.2020.12.010
发表时间：
2021-03-12
期刊：
Med (New York, N.Y.)
影响因子：
--
作者：
Vogels CBF;Watkins AE;Harden CA;Brackney DE;Shafer J;Wang J;Caraballo C;Kalinich CC;Ott IM;Fauver JR;Kudo E;Lu P;Venkataraman A;Tokuyama M;Moore AJ;Muenker MC;Casanovas-Massana A;Fournier J;Bermejo S;Campbell M;Datta R;Nelson A;Yale IMPACT Research Team;Dela Cruz CS;Ko AI;Iwasaki A;Krumholz HM;Matheus JD;Hui P;Liu C;Farhadian SF;Sikka R;Wyllie AL;Grubaugh ND
通讯作者：
Grubaugh ND
影响因子：
4.6
作者：
Wei S;Kohl E;Djandji A;Morgan S;Whittier S;Mansukhani M;Hod E;D'Alton M;Suh Y;Williams Z
通讯作者：
Williams Z