Early microbial succession in redeveloping dental biofilms in periodontal health and disease.
Early microbial succession in redeveloping dental biofilms in periodontal health and disease.
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DOI：
10.1111/j.1600-0765.2011.01409.x
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发表时间：
2012-02
影响因子：
3.5
通讯作者：
Haffajee AD
中科院分区：
文献类型：
--
作者：
Teles FR;Teles RP;Uzel NG;Song XQ;Torresyap G;Socransky SS;Haffajee AD
确定重新形成的牙龈上和牙龈下生物膜中细菌物种的继承顺序。分别从38名健康人和17名牙周炎患者的28颗牙齿中采集专业清洁后的龈上和龈下菌斑样本。在无口腔卫生的1、2、4和7天后，再次从随机选择的象限中的7颗牙齿中取样，并使用棋盘DNA-DNA杂交进行分析。在每个时间点对受试者内的%DNA探针计数取平均值。采用改进的移动窗口分析法确定生态演替。牙周炎和健康的龈上生物膜的演替是相似的。在第1天，缓症链球菌和粘膜奈瑟氏球菌的比例增加，其次是牙龈二氧化碳嗜纤维菌、腐蚀艾肯氏菌、小韦荣氏菌和口腔链球菌在1 - 4天。在第4 - 7天，直弯曲杆菌、Showae弯曲杆菌、产黑色素普雷沃菌和变黑普雷沃菌升高。龈下菌斑再形成较慢，与龈上菌斑有很大不同。首先观察到S的比例增加。缓症弧菌（V.mitis）、小弧菌（V.parvula）和C. gingivalis，以及在第7天由生痰二氧化碳嗜纤维菌和变黑丙酸杆菌引起。在任何临床组或位置中均未观察到牙周病原体比例的显著增加。在专业清洁后的早期龈上和龈下生物膜再发展中，细菌种类的演替有一个确定的顺序。
To determine the order of bacterial species succession in re-developing supra and subgingival biofilms. Supra and subgingival plaque samples were taken separately from 28 teeth in 38 healthy and 17 periodontitis subjects immediately after professional cleaning. Samples were taken again from 7 teeth in randomly selected quadrants after 1, 2, 4 and 7 days of no oral hygiene and analyzed using checkerboard DNA-DNA hybridization. % DNA probe counts were averaged within subjects at each time point. Ecological succession was determined using a modified moving window analysis. Succession in supragingival biofilms from periodontitis and health was similar. At 1 day, Streptococcus mitis and Neisseria mucosa showed increased proportions, followed by Capnocytophaga gingivalis, Eikenella corrodens, Veillonella parvula and Streptococcus oralis at 1–4 days. At 4–7 days, Campylobacter rectus, Campylobacter showae, Prevotella melaninogenica and Prevotella nigrescens became elevated. Subgingival plaque redevelopment was slower and very different from supragingival. Increased proportions were first observed for S. mitis, followed by V. parvula and C. gingivalis and, at 7 days by Capnocytophaga sputigena and P. nigrescens. No significant increase in proportions of periodontal pathogens was observed in any of the clinical groups or locations. There is a defined order in bacterial species succession in early supra and subgingival biofilm re-development after professional cleaning.
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影响因子：
14.9
作者：
Kolenbrander PE
通讯作者：
Kolenbrander PE
影响因子：
6.7
作者：
Haffajee, AD;Uzel, NG;Socransky, SS
通讯作者：
Socransky, SS
影响因子：
6.7
作者：
Marsh, Philip D.;Devine, Deirdre A.
通讯作者：
Devine, Deirdre A.
影响因子：
6.7
作者：
Cugini, MA;Haffajee, AD;Socransky, SS
通讯作者：
Socransky, SS
影响因子：
18.6
作者：
Haffajee, Anne D.;Teles, Ricardo P.;Socransky, Sigmund S.
通讯作者：
Socransky, Sigmund S.