Division site placement in E.coli: mutations that prevent formation of the MinE ring lead to loss of the normal midcell arrest of growth of polar MinD membrane domains.
Division site placement in E.coli: mutations that prevent formation of the MinE ring lead to loss of the normal midcell arrest of growth of polar MinD membrane domains.
复制标题
大肠杆菌中的分裂位点放置：阻止 MinE 环形成的突变导致极性 MinD 膜域生长的正常中细胞停滞的丧失。
DOI：
10.1093/emboj/cdf323
复制
发表时间：
2002
期刊：
影响因子：
--
通讯作者：
Rothfield,Lawrence
中科院分区：
文献类型：
--
作者：
Shih,Yu-Ling;Fu,Xiaoli;King,GlennF;Le,Trung;Rothfield,Lawrence
MinE蛋白作为拓扑特异性因子在大肠杆菌中决定隔膜位置。MinE在中细胞附近组装成膜相关环结构，并将MinD和MinC定位到膜相关极区，该极区经历特征性极间振荡周期。单（绿色荧光蛋白）和双标记（黄色荧光蛋白/青色荧光蛋白）荧光标记实验表明，MinE α面上位点的突变改变导致MinE环组装失败，与支持正常分裂位点放置模式的能力丧失相关。MinE环的缺失不会妨碍MinD极区的组装和拆卸。缺乏MinE环的突变细胞的特征在于MinD极区的生长超过其在中细胞附近的正常停滞点。结果表明，MinE环作为一种停止生长机制，防止MinCD极区延伸到中细胞分裂位点之外。
The MinE protein functions as a topological specificity factor in determining the site of septal placement in Escherichia coli. MinE assembles into a membrane‐associated ring structure near midcell and directs the localization of MinD and MinC into a membrane‐associated polar zone that undergoes a characteristic pole‐to‐pole oscillation cycle. Single (green fluorescent protein) and double label (yellow fluorescent protein/cyan fluorescent protein) fluorescence labeling experiments showed that mutational alteration of a site on the α‐face of MinE led to a failure to assemble the MinE ring, associated with loss of the ability to support a normal pattern of division site placement. The absence of the MinE ring did not prevent the assembly and disassembly of the MinD polar zone. Mutant cells lacking the MinE ring were characterized by the growth of MinD polar zones past their normal arrest point near midcell. The results suggested that the MinE ring acts as a stop‐growth mechanism to prevent the MinCD polar zone from extending beyond the midcell division site.
登录
查看更多内容
与多药耐药性相关的 170 至 180 千道尔顿膜糖蛋白的纯化。
影响因子：
4.8
作者：
Hirofumi Hamada;T. Tsuruo
通讯作者：
T. Tsuruo
P388 小鼠白血病的蒽环类药物耐药性及其钙拮抗剂的规避。
影响因子：
11.2
作者：
Kessel,D;Wilberding,C
通讯作者：
Wilberding,C
DOI：
10.1073/pnas.87.19.7777
发表时间：
1990-10-01
影响因子：
11.1
作者：
AMES, BN;PROFET, M;GOLD, LS
通讯作者：
GOLD, LS
溶剂 Cremophor EL 和 Tween 80 可调节多重耐药艾氏腹水肿瘤中柔红霉素的耐药性。
影响因子：
16.2
作者：
E. Friche;P. B. Jensen;M. Sehested;Demant Ej;Nissen Nn
通讯作者：
Nissen Nn
DOI：
10.1073/pnas.86.13.5128
发表时间：
1989-07-01
影响因子：
11.1
作者：
PEARCE, HL;SAFA, AR;BECK, WT
通讯作者：
BECK, WT