Effect of Metallothionein-III on Mercury-Induced Chemokine Gene Expression.
Effect of Metallothionein-III on Mercury-Induced Chemokine Gene Expression.
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DOI：
10.3390/toxics6030048
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发表时间：
2018-08-12
期刊：
影响因子：
4.6
通讯作者：
Satoh M
中科院分区：
文献类型：
--
作者：
Lee JY;Tokumoto M;Hwang GW;Kim MS;Takahashi T;Naganuma A;Yoshida M;Satoh M
已知汞化合物会导致中枢神经系统疾病;但其作用的详细分子机制仍不清楚。甲基汞会增加几种趋化因子基因的表达，特别是在大脑中，而金属硫蛋白III（MT-III）对各种脑部疾病具有保护作用。在这项研究中，我们调查了参与MT-III的趋化因子基因表达的变化，以应对甲基汞和汞蒸气在大脑和小脑的野生型小鼠和MT-III null小鼠。在所检查的任何脑组织中，未观察到野生型小鼠和MT-III缺失小鼠之间的汞浓度差异。Ccl 3在大脑和Cxcl 10在小脑的表达增加甲基汞在MT-III null，但不是野生型小鼠。Ccl 7在小脑中的表达增加了汞蒸气在MT-III null小鼠，但不是野生型小鼠。然而，Ccl 12和Cxcl 12的表达增加，在大脑中的甲基汞只有在野生型小鼠和Ccl 3的表达增加，在小脑中的汞蒸气只有在野生型小鼠。这些结果表明，MT-III不影响汞在大脑中的积累，但它会影响一些趋化因子基因的表达，以响应汞化合物。
Mercury compounds are known to cause central nervous system disorders; however the detailed molecular mechanisms of their actions remain unclear. Methylmercury increases the expression of several chemokine genes, specifically in the brain, while metallothionein-III (MT-III) has a protective role against various brain diseases. In this study, we investigated the involvement of MT-III in chemokine gene expression changes in response to methylmercury and mercury vapor in the cerebrum and cerebellum of wild-type mice and MT-III null mice. No difference in mercury concentration was observed between the wild-type mice and MT-III null mice in any brain tissue examined. The expression of Ccl3 in the cerebrum and of Cxcl10 in the cerebellum was increased by methylmercury in the MT-III null but not the wild-type mice. The expression of Ccl7 in the cerebellum was increased by mercury vapor in the MT-III null mice but not the wild-type mice. However, the expression of Ccl12 and Cxcl12 was increased in the cerebrum by methylmercury only in the wild-type mice and the expression of Ccl3 in the cerebellum was increased by mercury vapor only in the wild-type mice. These results indicate that MT-III does not affect mercury accumulation in the brain, but that it affects the expression of some chemokine genes in response to mercury compounds.
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影响因子：
2
作者：
Lee, Jin-Yong;Hwang, Gi-Wook;Naganuma, Akira
通讯作者：
Naganuma, Akira
影响因子：
2
作者：
Tokumoto, Maki;Lee, Jin-Yong;Satoh, Masahiko
通讯作者：
Satoh, Masahiko
影响因子：
6.1
作者：
Shimizu, Hidehisa;Bolati, Dilinaer;Niwa, Toshimitsu
通讯作者：
Niwa, Toshimitsu
影响因子：
4.6
作者：
Duerr GD;Dewald D;Schmitz EJ;Verfuerth L;Keppel K;Peigney C;Ghanem A;Welz A;Dewald O
通讯作者：
Dewald O
影响因子：
5.3
作者：
Gamo, Kazushige;Kiryu-Seo, Sumiko;Kiyama, Hiroshi
通讯作者：
Kiyama, Hiroshi