Maintaining replication origins in the face of genomic change.
Maintaining replication origins in the face of genomic change.
复制标题
DOI：
10.1101/gr.138248.112
复制
发表时间：
2012-10
期刊：
影响因子：
7
通讯作者：
Brewer BJ
中科院分区：
文献类型：
--
作者：
Di Rienzi SC;Lindstrom KC;Mann T;Noble WS;Raghuraman MK;Brewer BJ
对进化生物学家来说，复制的起源是一个悖论。作为一个集合，它们是绝对必要的基因组特征，但单独来说是高度多余和不必要的。因此，很难预测随着进化时间的推移，起源在多大程度上和在什么方面被保存下来。在这里，通过对发芽酵母Saccharmyces cerevisiae和Lachancea waltii的复制起点和染色体复制模式的比较基因组分析，我们评估了复制起点在多大程度上保留了1.5亿年进化产生的基因组变化。我们发现，华氏乳杆菌的起源表现出与酿酒酵母起源高度相似的核心共识序列和核小体占据模式。我们进一步观察到，沃尔蒂氏乳杆菌和酿酒酵母的染色体复制的总体进程是相似的。然而，几乎没有证据表明起源在两个物种之间的位置是保守的。在保守的起始点中，有那些围绕着丝粒和邻近组蛋白基因的起始点，这表明接近起始点可能对它们的调控很重要。我们的结论是，随着进化时间的推移，起源维持着序列、结构和调节，但不断地被创造和破坏，结果是它们的位置通常不被保存。
Origins of replication present a paradox to evolutionary biologists. As a collection, they are absolutely essential genomic features, but individually are highly redundant and nonessential. It is therefore difficult to predict to what extent and in what regard origins are conserved over evolutionary time. Here, through a comparative genomic analysis of replication origins and chromosomal replication patterns in the budding yeasts Saccharomyces cerevisiae and Lachancea waltii, we assess to what extent replication origins survived genomic change produced from 150 million years of evolution. We find that L. waltii origins exhibit a core consensus sequence and nucleosome occupancy pattern highly similar to those of S. cerevisiae origins. We further observe that the overall progression of chromosomal replication is similar between L. waltii and S. cerevisiae. Nevertheless, few origins show evidence of being conserved in location between the two species. Among the conserved origins are those surrounding centromeres and adjacent to histone genes, suggesting that proximity to an origin may be important for their regulation. We conclude that, over evolutionary time, origins maintain sequence, structure, and regulation, but are continually being created and destroyed, with the result that their locations are generally not conserved.
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影响因子：
14.9
作者：
Chang F;May CD;Hoggard T;Miller J;Fox CA;Weinreich M
通讯作者：
Weinreich M
影响因子：
10.5
作者：
Eaton, Matthew L.;Galani, Kyriaki;MacAlpine, David M.
通讯作者：
MacAlpine, David M.
DOI：
10.1038/nrm1663
发表时间：
2005-06
期刊：
Nature reviews. Molecular cell biology
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1093/bioinformatics/btn615
发表时间：
2009-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Carbon S;Ireland A;Mungall CJ;Shu S;Marshall B;Lewis S;AmiGO Hub;Web Presence Working Group
通讯作者：
Web Presence Working Group
影响因子：
5.3
作者：
Alvino, Gina M.;Collingwood, David;Raghuraman, M. K.
通讯作者：
Raghuraman, M. K.