Naotaifang extract treatment results in increased ferroportin expression in the hippocampus of rats subjected to cerebral ischemia.
Naotaifang extract treatment results in increased ferroportin expression in the hippocampus of rats subjected to cerebral ischemia.
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脑胎方提取物治疗导致脑缺血大鼠海马铁转运蛋白表达增加
DOI：
10.3892/mmr.2015.3309
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发表时间：
2015-06
影响因子：
3.4
通讯作者：
Ge JW
中科院分区：
文献类型：
--
作者：
Liao J;Xia X;Wang GZ;Shi YM;Ge JW
观察脑泰方治疗前后大鼠局灶性脑缺血后不同时间点膜铁转运蛋白（Fpn）的表达。首先，将大鼠随机分为大脑中动脉闭塞（MCAO）后2、6、12、24和72 h组，采用免疫组化和逆转录聚合酶链反应（RT-PCR）检测Fpn mRNA和蛋白水平。将大鼠随机分为假手术组、模型组、NTE低剂量组（3 g/kg）、NTE中剂量组（9 g/kg）和NTE高剂量组（27 g/kg）。每日1次灌胃给予相应治疗3 d后，采用MCAO线栓法复制局灶性脑缺血模型。第3天，采用神经行为学评估法对大鼠的神经行为学进行分析。免疫组化法检测海马CA 2区Fpn表达，RT-PCR法检测海马CA 2区Fpn mRNA表达。海马CA 2区Fpn表达于术后12 h达高峰（与模型组比较P<0.05）。NTE高剂量组（27 g/kg）神经行为学评分低于假手术组和模型组（P<0.05），Fpn mRNA和蛋白表达水平高于假手术组和模型组（P<0.05）。细胞内铁平衡失调可能是脑缺血的一种新机制。NTE可通过调节Fpn的表达来平衡脑缺血后海马CA 2区的铁水平，从而保护海马CA 2区的神经元。
The expression of Ferroportin (Fpn) was examined at different time points in rats following focal cerebral ischemia treated with or without the traditional Chinese medicine Naotaifang. Initially, rats were randomly divided into 2, 6, 12, 24 and 72 h groups following middle cerebral artery occlusion (MCAO) and the mRNA and protein level of Fpn was detected by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR) at the above time points. Secondly, the rats were randomly divided into five groups as follows: Sham surgery group, model group, low-dose group (3 g/kg NTE), medium dose group (9 g/kg NTE) and the high-dose group (27 g/kg NTE). After 3 days of corresponding therapy by intragastric administration once a day, the regional cerebral ischemia model was reproduced by the MCAO suture method. On the third day, the neurological behavior of the rats was analyzed by neurobehavioral assessment. Fpn in the hippocampal CA2 region was measured by immunohistochemistry and the mRNA level of Fpn was detected by RT-PCR. Expression of Fpn in the hippocampal CA2 region reached a peak 12 h after surgery (P<0.05, compared with the model group). The high-dose group (27 g/kg NTE) exhibited a lower neurological behavior score (P<0.05) and a higher level of expression of Fpn at the mRNA and protein level compared with the sham surgery group and model group (P<0.05). Dysregulation of intracellular iron balance is possibly a new mechanism underlying cerebral ischemia. NTE can protect the neuronal population in the hippocampal CA2 region by adjusting the expression of Fpn to balance iron levels following cerebral ischemia.
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