A novel multi-coaxial hollow fiber bioreactor for adherent cell types. Part 1: hydrodynamic studies.
A novel multi-coaxial hollow fiber bioreactor for adherent cell types. Part 1: hydrodynamic studies.
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一种用于贴壁细胞类型的新型多同轴中空纤维生物反应器。
DOI：
10.1002/bit.10081
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发表时间：
2002
影响因子：
3.8
通讯作者：
Macdonald,JeffreyM
中科院分区：
文献类型：
--
作者：
Wolfe,StephenP;Hsu,Edward;Reid,LolaM;Macdonald,JeffreyM
描述了一种用于贴壁细胞类型（例如肝脏）三维培养的新型多同轴生物反应器。它由四根直径逐渐增大的管子组成，一根一根放在另一根管子里面，形成四个空间隔离的隔间。通过将细胞夹在两个最里面的半透管或中空纤维之间的空间中，并产生从外室（ECC）穿过细胞团室到内室（ICC）的径向介质流，来模拟肝脏腺泡结构和生理参数。最外面的隔室由透气管形成，外壳用于将灌注介质充氧至 ECC 中的门静脉周围水平。使用蒸馏水进行实验，将径向流速 (Qr) 与 (1) 夹着细胞室的介质室之间的压降 (ΔP) 和 (2) 细胞室中的压力 (Pc) 相关联。将这些结果与根据最内层纤维的水渗透率计算的理论曲线进行比较。使用相衬速度编码磁共振成像直接可视化生物反应器内的轴向速度，并确认生物反应器中每个隔室边界处的层流和零轴向速度的假设。轴向流速是根据磁共振成像结果计算的，并且与先前描述的实验中测量的轴向流速相似。 © 2002 John Wiley & Sons, Inc. 生物技术 Bioeng 77: 83–90, 2002。
A novel multi‐coaxial bioreactor for three‐dimensional cultures of adherent cell types, such as liver, is described. It is composed of four tubes of increasing diameter placed one inside the other, creating four spatially isolated compartments. Liver acinar structure and physiological parameters are mimicked by sandwiching cells in the space between the two innermost semi‐permeable tubes, or hollows fibers, and creating a radial flow of media from an outer compartment (ECC), through the cell mass compartment, and to an inner compartment (ICC). The outermost compartment is created by gas‐permeable tubing, and the housing is used to oxygenate the perfusion media to periportal levels in the ECC. Experiments were performed using distilled water to correlate the radial flow rate (Qr) with (1) the pressure drop (ΔP) between the media compartments that sandwich the cell compartment and (2) the pressure in the cell compartment (Pc). These results were compared with the theoretical profile calculated based on the hydraulic permeability of the two innermost fibers. Phase–contrast velocity‐encoded magnetic resonance imaging was used to visualize directly the axial velocities inside the bioreactor and confirm the assumptions of laminar flow and zero axial velocity at the boundaries of each compartment in the bioreactor. Axial flow rates were calculated from the magnetic resonance imaging results and were similar to the measured axial flow rates for the previously described experiments. © 2002 John Wiley & Sons, Inc. Biotechnol Bioeng 77: 83–90, 2002.
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DOI：
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发表时间：
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