hUC-MSCs Exert a Neuroprotective Effect via Anti-apoptotic Mechanisms in a Neonatal HIE Rat Model
hUC-MSCs Exert a Neuroprotective Effect via Anti-apoptotic Mechanisms in a Neonatal HIE Rat Model
复制标题
hUC-MSCs 通过抗凋亡机制在新生 HIE 大鼠模型中发挥神经保护作用
DOI：
10.1177/0963689719874769
复制
发表时间：
2019-09
影响因子：
3.3
通讯作者：
Zhang Huanxiang
中科院分区：
文献类型：
--
作者：
Xu Jianwei;Feng Zhanhui;Wang Xianyao;Xiong Ying;Wang Lan;Ye Lan;Zhang Huanxiang
在这项研究中，我们研究了人脐带间充质干细胞如何通过抗凋亡机制在新生儿缺氧缺血性脑病大鼠模型中发挥神经保护作用。共使用78只10日龄（P10）大鼠。从人脐带中收集人脐带间充质干细胞并在培养中扩增后，在诱导缺氧缺血性脑病治疗后1小时将其给予大鼠受试者。采用神经行为功能评估方法评价人脐带间充质干细胞治疗后的短期（48 h）和长期（28 d）结局。在48 h时进行氯化三苯基四唑一水合物染色。Western blot和真实的实时聚合酶链反应检测Beclin-2和caspase-3在48 h的表达。收集人脐带间充质干细胞，经侧脑室注射给缺氧缺血性脑病幼仔。缺氧缺血性脑病通常会导致发育显著延迟，并导致大鼠受试者的认知和运动功能受损。人类脐带间充质干细胞被证明可以改善缺氧缺血性脑病引起的损伤，并改善认知和运动功能。虽然缺氧缺血性脑病诱导caspase-3和Beclin-2的表达，但人脐带间充质干细胞降低了这两种蛋白的表达。人脐带间充质干细胞可能作为一种潜在的治疗，以改善缺氧缺血性脑病患者的脑损伤。
In this study, we investigated how human umbilical cord mesenchymal stem cells exerted a neuroprotective effect via antiapoptotic mechanisms in a neonatal hypoxic-ischemic encephalopathy rat model. A total of 78 10-day old (P10) rats were used. After human umbilical cord mesenchymal stem cells were collected from human umbilical cords and amplified in culture, they were administered to rat subjects 1 h after induced hypoxic-ischemic encephalopathy treatment. The short-term (48 h) and long-term (28 day) outcomes were evaluated after human umbilical cord mesenchymal stem cells treatment using neurobehavioral function assessment. Triphenyltetrazolium chloride monohydrate staining was performed at 48 h. Beclin-2 and caspase-3 levels were evaluated with Western blot and real time polymerase chain reaction at 48 h. Human umbilical cord mesenchymal stem cells were collected and administrated to hypoxic-ischemic encephalopathy pups by intracerebroventricular injection. Hypoxic-ischemic encephalopathy typically induced significant delay in development and caused impairment in both cognitive and motor functions in rat subjects. Human umbilical cord mesenchymal stem cells were shown to ameliorate hypoxic-ischemic encephalopathy-induced damage and improve both cognitive and motor functions. Although hypoxic-ischemic encephalopathy induced significant expression of caspase-3 and Beclin-2, human umbilical cord mesenchymal stem cells decreased the expression of both of them. Human umbilical cord mesenchymal stem cells may serve as a potential treatment to ameliorate brain injury in hypoxic-ischemic encephalopathy patients.
登录
查看更多内容
Bcl-2 磷酸化触发自噬开关并减少缺血性中风后肢体远端缺血大鼠的线粒体损伤
影响因子：
6.9
作者：
Qi, Zhifeng;Dong, Wen;Luo, Yumin
通讯作者：
Luo, Yumin
影响因子：
6.9
作者：
Chen, Hank;Burris, Michael;Fajilan, Adrain;Spagnoli, Fred;Zhang, John H.;Tang, Jiping
通讯作者：
Tang, Jiping
DOI：
10.1074/jbc.m117.800813
发表时间：
2017-09
期刊：
The Journal of Biological Chemistry
影响因子：
--
作者：
S. Majdoul;J. Cosette;A. Seye;Eric Bernard;Sophie Frin;N. Holic;N. Chazal;L. Briant;L. Espert;A. Galy;D. Fenard
通讯作者：
S. Majdoul;J. Cosette;A. Seye;Eric Bernard;Sophie Frin;N. Holic;N. Chazal;L. Briant;L. Espert;A. Galy;D. Fenard
Beclin 2在自噬中功能，G蛋白偶联受体的降解和代谢。
影响因子：
64.5
作者：
He C;Wei Y;Sun K;Li B;Dong X;Zou Z;Liu Y;Kinch LN;Khan S;Sinha S;Xavier RJ;Grishin NV;Xiao G;Eskelinen EL;Scherer PE;Whistler JL;Levine B
通讯作者：
Levine B
基于氧化石墨烯辅助电化学信号放大的 caspase-3 活性检测的敏感细胞凋亡测定
影响因子：
12.6
作者：
Chen, Hongxia;Zhang, Jiangjiang;Yin, Yongmei
通讯作者：
Yin, Yongmei