PCR detection of structurally abnormal Y chromosomes
PCR detection of structurally abnormal Y chromosomes
复制标题
Y染色体结构异常的PCR检测
DOI：
10.1007/bf01900712
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Y. Nakagome
中科院分区：
文献类型：
--
作者：
S. Nagafuchi;S. Seki;Y. Nakahori;T. Tamura;H. Numabe;Y. Nakagome
对三个探针进行部分测序，每个探针检测Y染色体近端长臂上的基因座。因此，开发了3套新的引物，使这3个基因座的PCR检测。5个先前报道的引物组，3个在短臂上，每个在着丝粒和远端长臂上，与新的3个一起使用由8名患者组成的作图小组进行沿着作图，每个患者具有不同的Y染色体结构异常。现在，覆盖Y染色体全长的这8个位点的PCR检测已经成为可能，从而能够快速筛查Y染色体畸变患者。
Three probes each detecting a locus on the proximal long arm of the Y chromosome were partially sequenced. Thus, 3 sets of novel primers were developed which enable PCR detection of these 3 loci. Five previously reported primer sets, 3 on the short arm and each one on the centromere and the distal long arm, were mapped along with the novel three using a mapping panel consisted of 8 patients each with different structural abnormality of the Y chromosome. Now, PCR detection of these 8 loci covering an entire length of the Y chromosome has become possible enabling rapid screening of patients with Y chromosome aberrations.
影响因子：
64.8
作者：
SINCLAIR, AH;BERTA, P;GOODFELLOW, PN
通讯作者：
GOODFELLOW, PN