Observing the Assembly of Protein Complexes in Living Eukaryotic Cells in Super-Resolution Using refSOFI.
Observing the Assembly of Protein Complexes in Living Eukaryotic Cells in Super-Resolution Using refSOFI.
复制标题
使用 refSOFI 在超分辨率下观察活体真核细胞中蛋白质复合物的组装。
DOI:
10.1007/978-1-4939-7759-8_16
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发表时间:
2018
期刊:
影响因子:
--
通讯作者:
Zhang,Jin
中科院分区:
文献类型:
--
作者:
Hertel,Fabian;Mo,GaryCH;Dedecker,Peter;Zhang,Jin
Few approaches are currently available that allow the detection of protein-protein interactions (PPIs) in super-resolution, and the observation of the assembly of protein complexes in living cells has been particularly challenging. We developed reconstituted fluorescence-based stochastic optical fluctuation imaging (refSOFI), which is based on bimolecular fluorescence complementation (BiFC) and SOFI, allowing us to detect protein complex assembly 30 min after the induction of complex formation. Here we describe how to use refSOFI to map the assembly of two proteins of interest into a complex within living cells at super-resolution.
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DOI:
10.1016/0277-5379(88)90283-0
发表时间:
1988
期刊:
European journal of cancer & clinical oncology
影响因子:
--
作者:
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通讯作者:
J. Smyth
影响因子:
5.8
作者:
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通讯作者:
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5.8
作者:
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通讯作者:
Shinaberry,RG
影响因子:
11.2
作者:
R. Jaenke
通讯作者:
R. Jaenke
影响因子:
3.5
作者:
N. Bachur
通讯作者:
N. Bachur