Automated Method for Isolation of Adrenal Medullary Chromaffin Cells from Neonatal Porcine Glands
Automated Method for Isolation of Adrenal Medullary Chromaffin Cells from Neonatal Porcine Glands
复制标题
从新生猪腺中分离肾上腺髓质嗜铬细胞的自动化方法
DOI：
10.3727/000000001783986288
复制
发表时间：
2001
影响因子：
3.3
通讯作者：
J. Sagen
中科院分区：
文献类型：
--
作者：
C. Vizzardelli;E. Potter;T. Berney;A. Pileggi;L. Inverardi;C. Ricordi;J. Sagen
An automated method for the isolation of neonatal porcine adrenal chromaffin cells is described. Adrenal chromaffin cells are potentially useful for therapeutic transplantation, but current isolation methodology suffers from labor intensiveness and variability in yield and viability due to imprecision of manual techniques, enzyme purity, and gland age and species. The described approach utilizes an adaptation of an automated procedure previously described for isolation of pancreatic islets. Results from neonatal porcine adrenal glands revealed consistent cell yields with high (approximately 99%) viability. Catecholamine assays showed that the resultant cultures continue to produce and secrete norepinephrine and epinephrine. Immunocytochemical analysis indicated that the majority of cells in the preparation are chromaffin cells and adrenal cortical cells. The procedure meets the following requirements: 1) minimal traumatic action on the adrenal chromaffin cells, 2) continuous digestion in which the adrenal cells that are progressively liberated can be saved from further mechanical action, 3) minimal human intervention in the digestion process, and 4) high yield and viability of the isolated adrenal chromaffin cells.
体外培养和环孢素 A 处理对同种异体肾上腺髓质移植物存活的影响。
DOI：
10.1016/0022-4804(89)90042-5
发表时间：
1989
期刊：
The Journal of surgical research
影响因子：
--
作者：
Ricordi,C;Cryer,PE;Lacy,PE
通讯作者：
Lacy,PE
用于中枢神经系统移植的牛嗜铬细胞在体外不会引起异种 T 细胞增殖反应。
影响因子：
3.3
作者：
Czech,KA;Pollak,R;Pappas,GD;Sagen,J
通讯作者：
Sagen,J