Purification of 120,000 dalton envelope glycoprotein from culture fluids of human immunodeficiency virus (HIV)-infected H9 cells.
Purification of 120,000 dalton envelope glycoprotein from culture fluids of human immunodeficiency virus (HIV)-infected H9 cells.
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从人类免疫缺陷病毒 (HIV) 感染的 H9 细胞培养液中纯化 120,000 道尔顿包膜糖蛋白。
DOI：
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发表时间：
1987
影响因子：
1.5
通讯作者：
L. Arthur
中科院分区：
文献类型：
--
作者：
S. Pyle;J. Bess;W. Robey;P. Fischinger;R. Gilden;L. Arthur
已使用从AIDS患者血浆的免疫球蛋白制备的免疫亲和层析树脂从大体积（大于100升）HTLV-IIIB感染的H9细胞培养液中纯化了外包膜糖蛋白gp 120。通过使用一步免疫亲和纯化，从每升培养液中回收7至28微克的gp 120，其代表存在于培养液中的总包膜糖蛋白的60至95%。培养基中的包膜糖蛋白比起始材料浓缩10，000倍以上。含有痕量污染蛋白的水溶性gp 120通过制备型聚丙烯酰胺凝胶电泳（PAGE）纯化至表观均一性。从培养液中纯化的包膜糖蛋白在实验室动物中以天然形式和PAGE纯化形式具有免疫原性，并且在免疫测定中与AIDS患者血清反应。
The outer envelope glycoprotein, gp120, has been purified from large volumes (greater than 100 liters) of HTLV-IIIB-infected H9 cell culture fluids using immunoaffinity chromatography resins prepared from immunoglobulins of AIDS patients plasma. By using a single-step immunoaffinity purification, between 7 and 28 micrograms of gp120 was recovered from each liter of culture fluid which represented between 60 and 95% of the total envelope glycoprotein present in the fluid. Envelope glycoprotein in culture media was concentrated more than 10,000 times over the starting material. The water-soluble gp120, containing trace contaminating proteins, was purified to apparent homogeneity by preparative polyacrylamide gel electrophoresis (PAGE). Envelope glycoprotein purified from culture fluids was immunogenic in laboratory animals in both native and PAGE-purified forms and was reactive with AIDS patient sera in immunoassays.
预防人类免疫缺陷病毒感染的前景：纯化的120-kDa包膜糖蛋白诱导中和抗体。
DOI：
10.1073/pnas.83.18.7023
发表时间：
1986
影响因子：
11.1
作者：
Robey,WG;Arthur,LO;Matthews,TJ;Langlois,A;Copeland,TD;Lerche,NW;Oroszlan,S;Bolognesi,DP;Gilden,RV;Fischinger,PJ
通讯作者：
Fischinger,PJ
影响因子：
56.9
作者：
ALLAN, JS;COLIGAN, JE;ESSEX, M
通讯作者：
ESSEX, M
影响因子：
56.9
作者：
ROBEY, WG;SAFAI, B;FISCHINGER, PJ
通讯作者：
FISCHINGER, PJ