N-linked glycosylation is required for optimal function of Kaposi's sarcoma herpesvirus-encoded, but not cellular, interleukin 6.
N-linked glycosylation is required for optimal function of Kaposi's sarcoma herpesvirus-encoded, but not cellular, interleukin 6.
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DOI：
10.1084/jem.20031205
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发表时间：
2004-02-16
期刊：
影响因子：
--
通讯作者：
Miller G
中科院分区：
文献类型：
--
作者：
Dela Cruz CS;Lee Y;Viswanathan SR;El-Guindy AS;Gerlach J;Nikiforow S;Shedd D;Gradoville L;Miller G
卡波西肉瘤相关疱疹病毒白介素6(VIL-6)是人类细胞因子IL-6(hIL-6)的结构和功能同源物。IL-6和VIL-6具有相似的生物学功能，它们都通过gp130受体亚基激活Janus酪氨酸激酶(JAK)1和信号转导和转录激活因子(STAT)1/3通路。在这里，我们证明了VIL-6在N78和N89是N连接的糖基化的，并证明了VIL-6的N89位的N连接的糖基化显著地增强了与gp130的结合，通过JAK1-STAT1/3途径传递信号，并在细胞因子依赖的细胞增殖生物测定中发挥作用。虽然IL-6在N73位也是N-糖基化的，并且是O-糖基化的，但对于IL-6受体α依赖的gp130结合或通过JAK1-STAT1/3信号转导，非糖基化的hIL-6和糖基化的hIL-6在刺激B细胞增殖方面不同。这些发现突出了N-连接糖基化在病毒和细胞IL-6中的不同功能作用。
Kaposi's sarcoma–associated herpesvirus interleukin-6 (vIL-6) is a structural and functional homologue of the human cytokine IL-6 (hIL-6). hIL-6 and vIL-6 exhibit similar biological functions and both act via the gp130 receptor subunit to activate the Janus tyrosine kinase (JAK)1 and signal transducer and activator of transcription (STAT)1/3 pathway. Here we show that vIL-6 is N-linked glycosylated at N78 and N89 and demonstrate that N-linked glycosylation at site N89 of vIL-6 markedly enhances binding to gp130, signaling through the JAK1-STAT1/3 pathway and functions in a cytokine-dependent cell proliferation bioassay. Although hIL-6 is also N-glycosylated at N73 and multiply O-glycosylated, neither N-linked nor O-linked glycosylation is necessary for IL-6 receptor α–dependent binding to gp130 or signaling through JAK1-STAT1/3. As distinct from vIL-6, unglycosylated hIL-6 is as potent as glycosylated hIL-6 in stimulating B cell proliferation. These findings highlight distinct functional roles of N-linked glycosylation in viral and cellular IL-6.
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影响因子：
3.8
作者：
BOCK, GH;LONG, CA;NELSON, DL
通讯作者：
NELSON, DL
影响因子：
82.9
作者：
Nicholas, J;Ruvolo, VR;Reitz, MS
通讯作者：
Reitz, MS
影响因子：
56.9
作者：
Chatterjee, M;Osborne, J;Moore, PS
通讯作者：
Moore, PS
影响因子：
2.9
作者：
Chow, DC;Ho, J;Garcia, KC
通讯作者：
Garcia, KC
DOI：
10.1111/j.1432-1033.1993.tb17624.x
发表时间：
1993-02-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
KODAMA, S;TSUJIMOTO, M;KOBATA, A
通讯作者：
KOBATA, A