Proteolysis of mitotic chromosomes induces gradual and anisotropic decondensation correlated with a reduction of elastic modulus and structural sensitivity to rarely cutting restriction enzymes.
Proteolysis of mitotic chromosomes induces gradual and anisotropic decondensation correlated with a reduction of elastic modulus and structural sensitivity to rarely cutting restriction enzymes.
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有丝分裂染色体的蛋白水解诱导逐渐的各向异性解缩，与弹性模量的降低和对很少切割的限制性酶的结构敏感性相关。
DOI：
10.1091/mbc.e05-04-0321
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发表时间：
2005
影响因子：
3.3
通讯作者：
J. Marko
中科院分区：
文献类型：
--
作者：
L. H. Pope;Chee Xiong;J. Marko
用染色体弹性反应法研究了非特异性蛋白酶解对蝾螈单个染色体结构的影响。暴露于胰蛋白酶或蛋白酶K逐渐解除和软化染色体，但没有完全消除其弹性反应。染色体形态分析显示消化后各向异性解凝聚，长度增加超过宽度。长时间的蛋白酶处理只会导致染色体进一步肿胀，而不会完全溶解。轻度胰蛋白酶消化诱导染色体弹性对五个和六个碱基特异性限制酶的敏感性。这些结果，结合以前的研究核酸酶对有丝分裂染色体结构的影响，表明温和的蛋白水解逐渐降低有丝分裂染色体中的染色质约束元件的密度，提供了与有丝分裂染色体结构的各向异性折叠的“染色质网络”模型一致的证据。
The effect of nonspecific proteolysis on the structure of single isolated mitotic newt chromosomes was studied using chromosome elastic response as an assay. Exposure to either trypsin or proteinase K gradually decondensed and softened chromosomes but without entirely eliminating their elastic response. Analysis of chromosome morphology revealed anisotropic decondensation upon digestion, with length increasing more than width. Prolonged protease treatment resulted only in further swelling of the chromosome without complete dissolution. Mild trypsinization induced sensitivity of chromosome elasticity to five- and six-base-specific restriction enzymes. These results, combined with previous studies of effects of nucleases on mitotic chromosome structure, indicate that mild proteolysis gradually reduces the density of chromatin-constraining elements in the mitotic chromosome, providing evidence consistent with an anisotropically folded "chromatin network" model of mitotic chromosome architecture.
DOI：
10.1073/pnas.0402724101
发表时间：
2004-08-24
影响因子：
11.1
作者：
Kleckner, N;Zickler, D;Hutchinson, J
通讯作者：
Hutchinson, J
使用荧光原位杂交研究中期和间期染色体。
DOI：
10.1101/sqb.1993.058.01.084
发表时间：
1993
期刊：
Cold Spring Harbor symposia on quantitative biology
影响因子：
--
作者：
Trask,BJ;Allen,S;Massa,H;Fertitta,A;Sachs,R;vandenEngh,G;Wu,M
通讯作者：
Wu,M