Oxymatrine prevents NF-κB nuclear translocation and ameliorates acute intestinal inflammation.
Oxymatrine prevents NF-κB nuclear translocation and ameliorates acute intestinal inflammation.
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DOI：
10.1038/srep01629
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发表时间：
2013
影响因子：
4.6
通讯作者：
Jobin, Christian
中科院分区：
文献类型：
--
作者：
Guzman, Javier Rivera;Koo, Ja Seol;Goldsmith, Jason R.;Muehlbauer, Marcus;Narula, Acharan;Jobin, Christian
氧化苦参碱是一种传统的中草药产品，在心、脑和肝损伤模型中表现出抗炎作用。我们研究了氧化苦参碱在急性肠损伤和炎症模型中的作用。氧化苦参碱显著降低LPS诱导的NF-κ B驱动的荧光素酶活性，与大鼠IEC-6和小鼠BMDC中Cxcl 2、Tnfα和Il 6 mRNA表达的诱导减少相关。尽管氧化苦参碱降低LPS诱导的p65核转位和与Cxcl 2基因启动子的结合，但这种作用不依赖于IκBα降解/磷酸化。DSS诱导的体重减轻和组织学损伤在氧化苦参碱处理的C57 BL/6-WT小鼠中得到改善。虽然这种作用与结肠IL 6和IL 1 β mRNA积累减少相关，但在NF-κBEGFP小鼠中测量的总体NF-κB活性不受影响。我们的数据表明，氧化苦参碱减少LPS诱导的NF-κB核转位和活性独立于IκBα状态，通过阻断炎症信号传导预防肠道炎症，并改善体内整体肠道炎症。
Oxymatrine is a traditional Chinese herbal product that exhibits anti-inflammatory effects in models of heart, brain and liver injury. We investigated the impact of oxymatrine in an acute model of intestinal injury and inflammation. Oxymatrine significantly decreased LPS-induced NF-κB-driven luciferase activity, correlating with diminished induction of Cxcl2, Tnfα and Il6 mRNA expression in rat IEC-6 and murine BMDC. Although oxymatrine decreased LPS-induced p65 nuclear translocation and binding to the Cxcl2 gene promoter, this effect was independent of IκBα degradation/phosphorylation. DSS-induced weight loss and histological damage were ameliorated in oxymatrine-treated C57BL/6-WT-mice. While this effect correlated with reduced colonic Il6 and Il1β mRNA accumulation, global NF-κB activity as measured in NF-κBEGFP mice was unaffected. Our data demonstrate that oxymatrine reduces LPS-induced NF-κB nuclear translocation and activity independently of IκBα status, prevents intestinal inflammation through blockade of inflammatory signaling and ameliorates overall intestinal inflammation in vivo.
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影响因子：
29.4
作者：
Di Sabatino, Antonio;Pender, Sylvia L. F.;Macdonald, Thomas T.
通讯作者：
Macdonald, Thomas T.
影响因子：
4.8
作者：
Muehlbauer, Marcus;Chilton, Paula M.;Jobin, Christian
通讯作者：
Jobin, Christian
影响因子：
3.7
作者：
Joo YE;Karrasch T;Mühlbauer M;Allard B;Narula A;Herfarth HH;Jobin C
通讯作者：
Jobin C
影响因子：
7.2
作者：
Hong-li, Sun;Lei, Li;Bao-feng, Yang
通讯作者：
Bao-feng, Yang
DOI：
10.1152/ajpgi.90365.2008
发表时间：
2008-11-01
影响因子：
4.5
作者：
Larmonier, C. B.;Uno, J. K.;Kiela, P. R.
通讯作者：
Kiela, P. R.