Effect of Gegen Qinlian Decoction on Cardiac Gene Expression in Diabetic Mice.
Effect of Gegen Qinlian Decoction on Cardiac Gene Expression in Diabetic Mice.
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DOI：
10.1155/2017/7421761
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发表时间：
2017
影响因子：
2.9
通讯作者：
Wu Y
中科院分区：
文献类型：
--
作者：
Han J;Wang Z;Xing W;Yuan Y;Zhang Y;Lv T;Wang H;Liu Y;Wu Y
本研究的目的是观察GGQL汤对心功能不全的治疗作用，并阐明其药理作用机制。db/db小鼠分为DB组和GGQL组，WT小鼠作为对照。通过超声心动图检查所有小鼠。并对LV组织标本进行总RNA测序，分析差异表达基因。通过4个鉴定为差异表达的基因的RT-qPCR结果验证RNA-seq结果。测定心肌组织中丙酮酸和神经酰胺的含量。结果表明，GGQL汤能显著改善舒张功能障碍，增加丙酮酸含量，并有降低神经酰胺含量的趋势。RNA-seq结果显示，DB组与WT组相比，有2958个基因差异表达。其中，与DB组相比，GGQL组中有26个基因差异表达。4个基因的表达结果与RNA-seq结果一致。本研究结果提示，GGQL汤对左室舒张功能不全有一定的治疗作用，其作用可能与其促进心肌糖酵解、降低神经酰胺含量有关。
The aim of this research is to investigate the therapeutic effect of GGQL decoction on cardiac dysfunction and elucidate the pharmacological mechanisms. db/db mice were divided into DB group or GGQL group, and WT mice were used as control. All mice were accessed by echocardiography. And the total RNA of LV tissue samples was sequenced, then differential expression genes were analyzed. The RNA-seq results were validated by the results of RT-qPCR of 4 genes identified as differentially expressed. The content of pyruvate and ceramide in myocardial tissue was also measured. The results showed that GGQL decoction could significantly improve the diastolic dysfunction, increase the content of pyruvate, and had the trend to reduce the ceramide content. The results of RNA-seq showed that 2958 genes were differentially expressed when comparing the DB group with the WT group. Among them, compared with the DB group, 26 genes were differentially regulated in the GGQL group. The expression results of 4 genes were consistent with the RNA-seq results. Our study reveals that GGQL decoction has a therapeutic effect on diastolic dysfunction of the left ventricular and the effect may be related to its role in promoting myocardial glycolysis and decreasing the content of ceramide.
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影响因子：
7.2
作者：
Li, Huan-Ting;Wu, Xiao-Dong;Wang, Jiping
通讯作者：
Wang, Jiping
DOI：
10.1152/ajpendo.2000.279.5.e1104
发表时间：
2000-11-01
影响因子：
5.1
作者：
Belke, DD;Larsen, TS;Severson, DL
通讯作者：
Severson, DL
DOI：
10.1152/ajpheart.00452.2009
发表时间：
2009-12-01
影响因子：
4.8
作者：
Basu, Ratnadeep;Oudit, Gavin Y.;Kassiri, Zamaneh
通讯作者：
Kassiri, Zamaneh
影响因子：
3.7
作者：
Chen WC;Kawasaki N;Nycholat CM;Han S;Pilotte J;Crocker PR;Paulson JC
通讯作者：
Paulson JC
影响因子：
3.7
作者：
Mizutani Y;Sun H;Ohno Y;Sassa T;Wakashima T;Obara M;Yuyama K;Kihara A;Igarashi Y
通讯作者：
Igarashi Y