The effects of benzo[a]pyrene on leucocyte distribution and antibody response in rainbow trout (Oncorhynchus mykiss).
The effects of benzo[a]pyrene on leucocyte distribution and antibody response in rainbow trout (Oncorhynchus mykiss).
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苯并[a]芘对虹鳟鱼（Oncorhynchus mykiss）白细胞分布和抗体反应的影响。
DOI：
10.1016/j.aquatox.2013.12.017
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发表时间：
2014
影响因子：
4.5
通讯作者：
M. R. van den Heuvel
中科院分区：
文献类型：
--
作者：
L. Phalen;B. Köllner;L. A. Leclair;N. Hogan;M. R. van den Heuvel
多环芳烃（PAHs）是一组具有免疫毒性和致癌潜力的化合物，可能对鱼类种群构成威胁。本研究以苯并[a]芘（BaP）为代表性免疫毒性多环芳烃（PAH），利用新开发的鱼类免疫毒理学模型，研究了多环芳烃对虹鳟鱼免疫组织/细胞群体水平的影响。腹腔注射25或100 mg/kg BaP导致持续暴露，如BaP波长下胆汁荧光所示，持续暴露长达42天。建立了一种新的流式细胞术方法，该方法将组织中总白细胞的绝对定量计数（3，3 ′-dihexyloxacarbocyanine iodide（DiOC 6）染料）与B细胞、T细胞、髓样细胞和血小板单克隆抗体的相对分类计数相结合，用于脾、血液和头肾中白细胞分类分布的绝对计数。实验表明，在接触7、14或21天后，血液、脾脏或头肾中的B细胞、骨髓细胞或T细胞的绝对数量呈剂量和时间依赖性减少。任何组织中红细胞或血小板的绝对数量均无变化。虹鳟鱼暴露于100 mg/kg BaP 21 d后，再暴露于灭活的杀鲑气单胞菌，其循环抗体浓度下降了56%。结论BaP对虹鳟鱼的某些免疫细胞具有细胞系特异性毒性作用，并导致循环抗体水平下降。
Polycyclic aromatic hydrocarbons (PAHs) are a group of compounds with immunotoxic and carcinogenic potential that may pose a threat to fish populations. This study aims to utilize a newly developed fish immunotoxicology model to determine the immune tissue/cell population level effects of PAHs on rainbow trout, using benzo[a]pyrene (BaP) as a representative immunotoxic PAH. Intraperitoneal injection of 25 or 100 mg/kg BaP resulted in sustained exposure as indicated by biliary fluorescence at BaP wavelengths for up to 42 days. A new flow cytometry method for absolute counts of differential leucocyte distributions in spleen, blood, and head kidney was developed by combining absolute quantitative counts of total leukocytes in the tissue (3,3′-dihexyloxacarbocyanine iodide (DiOC6) dye) with relative differential counts using monoclonal antibodies for B cells, T cells, myeloid cells, and thrombocytes. Experiments indicated dose- and time-dependent decreases in the absolute number of B cells, myeloid cells, or T cells in blood, spleen, or head kidney after 7, 14 or 21 d of exposure. There was no change in the absolute numbers of erythrocytes or thrombocytes in any tissue. When rainbow trout were exposed to inactivatedAeromonas salmonicidaafter a 21 d exposure to 100 mg/kg BaP, circulating antibody concentrations were decreased by 56%. It was concluded that BaP has a cell lineage-specific toxic effect on some immune cells of rainbow trout, and causes a decrease in circulating antibody levels.
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影响因子：
6.8
作者：
McNeill, Sean A.;Arens, Collin J.;van den Heuvel, Michael R.
通讯作者：
van den Heuvel, Michael R.
致癌物苯并(a)芘可直接抑制小鼠脾细胞的体外抗体产生，但非致癌同系物苯并(e)芘则不会直接抑制。
影响因子：
11.2
作者：
WhiteJr,KL;Holsapple,MP
通讯作者：
Holsapple,MP
影响因子：
3.8
作者：
Gao, J;Lauer, FT;Burchiel, SW
通讯作者：
Burchiel, SW
影响因子：
3.8
作者：
Curtis LR;Garzon CB;Arkoosh M;Collier T;Myers MS;Buzitis J;Hahn ME
通讯作者：
Hahn ME
影响因子：
3.8
作者：
Davila, DR;Romero, DL;Burchiel, SW
通讯作者：
Burchiel, SW