The genome and transcriptome of Haemonchus contortus, a key model parasite for drug and vaccine discovery.
The genome and transcriptome of Haemonchus contortus, a key model parasite for drug and vaccine discovery.
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DOI：
10.1186/gb-2013-14-8-r88
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发表时间：
2013-08-28
期刊：
影响因子：
12.3
通讯作者：
Cotton JA
中科院分区：
文献类型：
--
作者：
Laing R;Kikuchi T;Martinelli A;Tsai IJ;Beech RN;Redman E;Holroyd N;Bartley DJ;Beasley H;Britton C;Curran D;Devaney E;Gilabert A;Hunt M;Jackson F;Johnston SL;Kryukov I;Li K;Morrison AA;Reid AJ;Sargison N;Saunders GI;Wasmuth JD;Wolstenholme A;Berriman M;Gilleard JS;Cotton JA
小反刍动物弓形血线虫是药物发现、疫苗开发和驱虫耐药性研究中应用最广泛的寄生虫。它产生耐药性的显著倾向威胁到世界许多地区绵羊产业的生存能力，并提供了大量给药控制寄生线虫效果的一个警示性例子。它的系统发育位置使它特别适合与自由生活的线虫秀丽隐杆线虫和家畜和人类最重要的经济寄生虫进行比较。在这里，我们报告了一个详细的分析草案基因组组装和广泛的转录组数据集的H.扭曲。这是迄今为止首次发表的强体线虫基因组，也是迄今为止报道的最广泛的寄生线虫转录组数据集。我们展示了弯曲线虫和秀丽隐杆线虫之间基因组结构和基因含量的一般保守模式，以及重要寄生虫基因家族的急剧扩张。我们确定了参与寄生虫特异性途径的基因，如血液摄食、神经功能和药物代谢。特别是，我们描述了已知药物靶家族的完整基因库，提供了对几种重要驱虫药作用的最全面的了解。此外，我们确定了一组在生命周期的寄生阶段和寄生虫肠道中富集的基因，这些基因为疫苗和药物候选靶点提供了丰富的来源。弓形虫的基因组和转录组为弓形虫和其他重要的硬柱体寄生虫的后基因组研究提供了重要的平台。
The small ruminant parasite Haemonchus contortus is the most widely used parasitic nematode in drug discovery, vaccine development and anthelmintic resistance research. Its remarkable propensity to develop resistance threatens the viability of the sheep industry in many regions of the world and provides a cautionary example of the effect of mass drug administration to control parasitic nematodes. Its phylogenetic position makes it particularly well placed for comparison with the free-living nematode Caenorhabditis elegans and the most economically important parasites of livestock and humans. Here we report the detailed analysis of a draft genome assembly and extensive transcriptomic dataset for H. contortus. This represents the first genome to be published for a strongylid nematode and the most extensive transcriptomic dataset for any parasitic nematode reported to date. We show a general pattern of conservation of genome structure and gene content between H. contortus and C. elegans, but also a dramatic expansion of important parasite gene families. We identify genes involved in parasite-specific pathways such as blood feeding, neurological function, and drug metabolism. In particular, we describe complete gene repertoires for known drug target families, providing the most comprehensive understanding yet of the action of several important anthelmintics. Also, we identify a set of genes enriched in the parasitic stages of the lifecycle and the parasite gut that provide a rich source of vaccine and drug target candidates. The H. contortus genome and transcriptome provide an essential platform for postgenomic research in this and other important strongylid parasites.
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影响因子：
1.5
作者：
Forrester, SG;Prichard, RK;Beech, RN
通讯作者：
Beech, RN
影响因子：
5.4
作者：
Fuchs S;Bundy JG;Davies SK;Viney JM;Swire JS;Leroi AM
通讯作者：
Leroi AM
影响因子：
30.8
作者：
Dieterich, Christoph;Clifton, Sandra W.;Schuster, Lisa N.;Chinwalla, Asif;Delehaunty, Kimberly;Dinkelacker, Iris;Fulton, Lucinda;Fulton, Robert;Godfrey, Jennifer;Minx, Pat;Mitreva, Makedonka;Roeseler, Waltraud;Tian, Huiyu;Witte, Hanh;Yang, Shiaw-Pyng;Wilson, Richard K.;Sommer, Ralf J.
通讯作者：
Sommer, Ralf J.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
DOI：
10.1073/pnas.0806933105
发表时间：
2008-11-25
影响因子：
11.1
作者：
Boulin, Thomas;Gielen, Marc;Bessereau, Jean-Louis
通讯作者：
Bessereau, Jean-Louis