Gene silencing of BnTT10 family genes causes retarded pigmentation and lignin reduction in the seed coat of Brassica napus.
Gene silencing of BnTT10 family genes causes retarded pigmentation and lignin reduction in the seed coat of Brassica napus.
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BnTT10 家族基因的基因沉默导致甘蓝型油菜种皮色素沉着延迟和木质素减少
DOI：
10.1371/journal.pone.0061247
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Zhang K;Lu K;Qu C;Liang Y;Wang R;Chai Y;Li J
黄籽（即黄种皮）是芸苔属植物最重要的农艺性状之一，与籽油和籽粕品质有关。以往对拟南芥和芸苔科植物的研究表明，种子颜色性状在分子水平上与类黄酮和木质素的生物合成有关。在拟南芥中，类黄酮的氧化聚合和木质素的生物合成被证明是由AtTT10基因编码的功能酶漆酶15催化的。本研究从油菜中分离出3个TT10基因，从油菜中分离出3个TT10基因，从甘蓝中分离出2个TT10基因，并对其在类黄酮氧化/聚合和木质素生物合成中的作用进行了研究。根据我们的系统发育分析，这些基因可以分为两类，在结构和功能上有明显的差异。表达研究表明，TT10基因在油菜种子发育过程中具有活性，但在黄籽和黑籽近等基因系中表达模式存在差异。为进行功能分析，选择3个黑籽甘蓝型油菜品种进行转基因研究。表达反义TT10构建体的转基因甘蓝型油菜种皮色素沉着迟缓。化学成分分析显示，与对照相比，这些转基因植物种皮中可溶性原花青素含量增加，可提取木质素含量降低。这些发现提示TT10基因在甘蓝型油菜原花青素聚合和木质素生物合成以及种皮色素沉着中发挥作用。
Yellow-seed (i.e., yellow seed coat) is one of the most important agronomic traits of Brassica plants, which is correlated with seed oil and meal qualities. Previous studies on the Brassicaceae, including Arabidopsis and Brassica species, proposed that the seed-color trait is correlative to flavonoid and lignin biosynthesis, at the molecular level. In Arabidopsis thaliana, the oxidative polymerization of flavonoid and biosynthesis of lignin has been demonstrated to be catalyzed by laccase 15, a functional enzyme encoded by the AtTT10 gene. In this study, eight Brassica TT10 genes (three from B. napus, three from B. rapa and two from B. oleracea) were isolated and their roles in flavonoid oxidation/polymerization and lignin biosynthesis were investigated. Based on our phylogenetic analysis, these genes could be divided into two groups with obvious structural and functional differentiation. Expression studies showed that Brassica TT10 genes are active in developing seeds, but with differential expression patterns in yellow- and black-seeded near-isogenic lines. For functional analyses, three black-seeded B. napus cultivars were chosen for transgenic studies. Transgenic B. napus plants expressing antisense TT10 constructs exhibited retarded pigmentation in the seed coat. Chemical composition analysis revealed increased levels of soluble proanthocyanidins, and decreased extractable lignin in the seed coats of these transgenic plants compared with that of the controls. These findings indicate a role for the Brassica TT10 genes in proanthocyanidin polymerization and lignin biosynthesis, as well as seed coat pigmentation in B. napus.
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影响因子：
12.3
作者：
Mun JH;Kwon SJ;Yang TJ;Seol YJ;Jin M;Kim JA;Lim MH;Kim JS;Baek S;Choi BS;Yu HJ;Kim DS;Kim N;Lim KB;Lee SI;Hahn JH;Lim YP;Bancroft I;Park BS
通讯作者：
Park BS
影响因子：
4.3
作者：
Liang, Mingxiang;Davis, Elizabeth;Wu, Yajun
通讯作者：
Wu, Yajun
影响因子：
6.2
作者：
Cardoza, V;Stewart, CN
通讯作者：
Stewart, CN
来自甘蓝型油菜和亲本物种的透明测试 12 基因：克隆、进化和黄色种子性状的差异参与
影响因子：
3.1
作者：
Chai, You-Rong;Lei, Bo;Chen, Li
通讯作者：
Chen, Li
影响因子：
4.3
作者：
McCaig, BC;Meagher, RB;Dean, JFD
通讯作者：
Dean, JFD