Isolation and characterization of the gene associated with geosmin production in cyanobacteria.
Isolation and characterization of the gene associated with geosmin production in cyanobacteria.
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DOI：
10.1021/es801465w
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发表时间：
2008-11-01
影响因子：
11.4
通讯作者：
Monis, Paul T.
中科院分区：
文献类型：
--
作者：
Giglio, Steven;Jiang, Jiaoyang;Saint, Christopher P.;Cane, David E.;Monis, Paul T.
Geosmin是一种次生代谢物，在饮用水供应中会产生泥土味和气味。Geosmin仍然是对自来水公司管理制度的挑战，仍然是消费者投诉的最常见原因之一，因为“脏”水的味道可能表明消毒制度失败，水可能不安全饮用。尽管蓝藻被报道对这些味道和气味事件负有主要责任，但对于Geosmin是如何产生的，或者为什么产生，研究人员一直没有找到答案。在这里，我们首次描述了在一个模型蓝藻，点状念珠藻73102(Atcc 29133)中产生土豆蛋白的机制，我们证明了它利用单一的酶催化法尼基二磷酸环化成土豆蛋白。利用这些信息，我们开发了一种基于聚合酶链式反应的分析方法，可以快速检测产生土豆凝集素的蓝藻。这项测试可以用来确认和跟踪任何给定水体中是否出现产生味道和气味的蓝藻，因此可以作为可能遭受不良味道和气味事件的水体管理人员的早期预警系统。
Geosmin is a secondary metabolite responsible for earthy tastes and odors in potable water supplies. Geosmin continues to be a challenge to water utility management regimes and remains one of the most common causes of consumer complaints, as the taste of “dirty” water may suggest a failed disinfection regime and that the water may be unsafe to drink. Although cyanobacteria have been reported to be largely responsible for these taste and odor events, the answer as to how or why geosmin is produced has eluded researchers. We describe here for the first time the mechanism by which geosmin is produced in a model cyanobacterium, Nostoc punctiforme PCC 73102 (ATCC 29133), which we demonstrate utilizes a single enzyme to catalyze the cyclization of farnesyl diphosphate to geosmin. Using this information, we have developed a PCR-based assay that allows the rapid detection of geosmin-producing cyanobacteria. This test may be utilized to confirm and track the emergence of taste and odor-producing cyanobacteria in any given water body and thus can be used as an early warning system by managers of water bodies that may suffer from adverse taste and odor episodes.
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影响因子：
8.8
作者：
Ho, Lionel;Hoefel, Daniel;Newcombe, Gayle
通讯作者：
Newcombe, Gayle
影响因子：
2.7
作者：
IZAGUIRRE, G;TAYLOR, WD
通讯作者：
TAYLOR, WD
影响因子：
2.1
作者：
Schulz, S;Fuhlendorff, J;Reichenbach, H
通讯作者：
Reichenbach, H
影响因子：
14.8
作者：
Jiang, Jiaoyang;He, Xiaofei;Cane, David E.
通讯作者：
Cane, David E.
DOI：
10.1073/pnas.0337542100
发表时间：
2003-02-18
影响因子：
11.1
作者：
Gust, B;Challis, GL;Chater, KF
通讯作者：
Chater, KF