Refinement of Immunohistologic Parameters for Her2/neu Scoring Validation by FISH and CISH
Refinement of Immunohistologic Parameters for Her2/neu Scoring Validation by FISH and CISH
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通过 FISH 和 CISH 改进 Her2/neu 评分验证的免疫组织学参数
DOI：
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
A. Morey
中科院分区：
文献类型：
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作者：
A. Leong;M. Formby;Z. Haffajee;M. Clarke;A. Morey
与荧光原位杂交（FISH）获得的结果相比，Her 2/neu免疫染色评分的常规方法被认为在2+病例中导致较高的假阳性率;然而，成本和便利性决定了免疫组织化学仍然是乳腺癌患者Her 2/neu状态的筛查试验。我们描述了精细的评分标准Her 2/neu的基础上的解剖定位，而不是主观评估的强度。对于3+评分（Her 2/neu过表达），由>25%的肿瘤细胞中的并置细胞膜染色导致的周向电车轨道模式的存在是必要的，并且<25%的电车轨道模式的存在评分为2+（不确定）;颗粒和碎片膜染色评分为1+（阴性）。Her 2/neu过表达的电车轨道模式显示与基因扩增100%一致。在其他类别的选定病例中进行FISH和CISH检测，验证了修订后的评分方法。这些标准减少了需要FISH检测的可疑染色病例的数量。
The conventional method of scoring Her2/neu immunostaining is recognized to result in a high false-positive rate among 2+ cases when compared with results obtained with fluorescence in situ hybridization (FISH); however, costs and convenience dictates that immunohistochemistry remains the screening test for Her2/neu status in patients with breast cancer. We describe refined criteria for scoring of Her2/neu on the basis of anatomic localization rather than the subjective assessment of intensity. The presence of a circumferential tram track pattern that results from the staining of apposing cell membranes in >25% of the tumor cells was necessary for a 3+ score (Her2/neu overexpressed) and the presence of the tram track pattern in <25% was scored 2+ (equivocal); granular and fragmented membrane staining was scored 1+ (negative). The tram track pattern of Her2/neu overexpression showed 100% concordance with gene amplification. FISH and CISH testing in selected cases from the other categories validated the revised scoring method. These criteria reduced the numbers of equivocal staining cases that required FISH testing.
影响因子：
158.5
作者：
Romond, EH;Perez, EA;Wolmark, N
通讯作者：
Wolmark, N
影响因子：
3.7
作者：
Deng, Jingyu;Liang, Han;Liu, Xiangyu
通讯作者：
Liu, Xiangyu
档案组织样本中 HER-2/neu 抗体的敏感性：癌基因表达免疫组织化学研究中潜在的错误来源。
影响因子：
11.2
作者：
Press,MF;Hung,G;Godolphin,W;Slamon,DJ
通讯作者：
Slamon,DJ