A physical and genetic map of Cannabis sativa identifies extensive rearrangements at the THC/CBD acid synthase loci.
A physical and genetic map of Cannabis sativa identifies extensive rearrangements at the THC/CBD acid synthase loci.
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DOI：
10.1101/gr.242594.118
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发表时间：
2019-01
期刊：
影响因子：
7
通讯作者：
van Bakel H
中科院分区：
文献类型：
--
作者：
Laverty KU;Stout JM;Sullivan MJ;Shah H;Gill N;Holbrook L;Deikus G;Sebra R;Hughes TR;Page JE;van Bakel H
大麻被广泛种植用于医疗，食品，工业和娱乐用途，但关于其遗传学，包括大麻素含量的分子决定因素，仍有许多未知之处。在这里，我们描述了一个组合的物理和遗传图谱来自毒品型菌株紫色库什和大麻品种之间的交叉“Finola”。该图谱显示，大麻素生物合成基因通常是不相关的，但产生THCA和CBDA脱氢酶（THCAS和CBDAS）底物的芳香族异戊二烯基转移酶（AP）与总大麻素含量的已知标记紧密相关。我们进一步确定了编码CBCA合成酶（CBCAS）的基因，并表征了其催化活性，从而深入了解大麻中大麻素的多样性。THCAS和CBDAS（其确定药物与大麻化学型）包含在大的（>250 kb）富含反转录转座子的区域内，所述区域在药物型和大麻型等位基因之间高度非同源，并且还嵌入在1040 Mb的最小重组重复DNA内。染色体结构与小麦等谷物中的染色体结构相似，重组集中在染色体末端附近的基因丰富、重复缺失的区域。物理和遗传图谱应有助于进一步剖析这种具有商业和医学重要性的植物的遗传和分子机制。
Cannabis sativa is widely cultivated for medicinal, food, industrial, and recreational use, but much remains unknown regarding its genetics, including the molecular determinants of cannabinoid content. Here, we describe a combined physical and genetic map derived from a cross between the drug-type strain Purple Kush and the hemp variety “Finola.” The map reveals that cannabinoid biosynthesis genes are generally unlinked but that aromatic prenyltransferase (AP), which produces the substrate for THCA and CBDA synthases (THCAS and CBDAS), is tightly linked to a known marker for total cannabinoid content. We further identify the gene encoding CBCA synthase (CBCAS) and characterize its catalytic activity, providing insight into how cannabinoid diversity arises in cannabis. THCAS and CBDAS (which determine the drug vs. hemp chemotype) are contained within large (>250 kb) retrotransposon-rich regions that are highly nonhomologous between drug- and hemp-type alleles and are furthermore embedded within ∼40 Mb of minimally recombining repetitive DNA. The chromosome structures are similar to those in grains such as wheat, with recombination focused in gene-rich, repeat-depleted regions near chromosome ends. The physical and genetic map should facilitate further dissection of genetic and molecular mechanisms in this commercially and medically important plant.
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影响因子：
4.5
作者：
Liu S;Yeh CT;Ji T;Ying K;Wu H;Tang HM;Fu Y;Nettleton D;Schnable PS
通讯作者：
Schnable PS
影响因子：
30.8
作者：
Choi, Kyuha;Zhao, Xiaohui;Kelly, Krystyna A.;Venn, Oliver;Higgins, James D.;Yelina, Nataliya E.;Hardcastle, Thomas J.;Ziolkowski, Piotr A.;Copenhaver, Gregory P.;Franklin, F. Chris H.;McVean, Gil;Henderson, Ian R.
通讯作者：
Henderson, Ian R.
影响因子：
2.2
作者：
Kojoma, Mareshige;Seki, Hikaru;Muranaka, Toshiya
通讯作者：
Muranaka, Toshiya
影响因子：
7.3
作者：
Maione, Sabatino;Piscitelli, Fabiana;Di Marzo, Vincenzo
通讯作者：
Di Marzo, Vincenzo
影响因子：
48
作者：
Chin, Chen-Shan;Peluso, Paul;Schatz, Michael C.
通讯作者：
Schatz, Michael C.