Structure and mechanism of the Red recombination system of bacteriophage λ.
Structure and mechanism of the Red recombination system of bacteriophage λ.
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DOI：
10.1016/j.pbiomolbio.2019.03.005
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发表时间：
2019-10
影响因子：
3.8
通讯作者：
Bell CE
中科院分区：
文献类型：
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作者：
Caldwell BJ;Bell CE
虽然这卷的大部分集中在直接参与基因组稳定性和癌症的哺乳动物DNA修复系统，但重要的是仍然要注意原核生物的模型系统。本文综述了噬菌体λ的Red重组系统，该系统由切割dsDNA末端的核酸外切酶和结合产生的3′-突出端并使其与互补链退火的单链退火蛋白（SSAP）组成。Red的遗传学和生物化学已经研究了50多年，为理解高等真核生物中的DNA重组奠定了基础。事实上，Red核酸外切酶（λ exo）与真核生物中参与DNA末端切除的核酸酶Dna 2同源，Red退火蛋白（Redβ）与真核生物中主要的SSAP Rad 52同源。虽然真核重组涉及一个复杂的蛋白质网络，但仍在解开，噬菌体系统相对简单和流线型，但仍然包含重组的基本特征，即DNA末端切除，同源配对（退火），以及它们之间的耦合。此外，Red系统已被用于功能基因组学和系统生物学重要的细菌基因组工程的强大方法中。然而，Red的几个机制方面，特别是退火蛋白的作用，仍然知之甚少。这篇评论将集中在红色重组系统的蛋白质，特别注意结构和机制方面，以及如何吸取的教训可以应用到真核系统。
While much of this volume focuses on mammalian DNA repair systems that are directly involved in genome stability and cancer, it is important to still be mindful of model systems from prokaryotes. Herein we review the Red recombination system of bacteriophage λ, which consists of an exonuclease for resecting dsDNA ends, and a single-strand annealing protein (SSAP) for binding the resulting 3′-overhang and annealing it to a complementary strand. The genetics and biochemistry of Red have been studied for over 50 years, in work that has laid much of the foundation for understanding DNA recombination in higher eukaryotes. In fact, the Red exonuclease (λ exo) is homologous to Dna2, a nuclease involved in DNA end-resection in eukaryotes, and the Red annealing protein (Redβ) is homologous to Rad52, the primary SSAP in eukaryotes. While eukaryotic recombination involves an elaborate network of proteins that is still being unraveled, the phage systems are comparatively simple and streamlined, yet still encompass the fundamental features of recombination, namely DNA end-resection, homologous pairing (annealing), and a coupling between them. Moreover, the Red system has been exploited in powerful methods for bacterial genome engineering that are important for functional genomics and systems biology. However, several mechanistic aspects of Red, particularly the action of the annealing protein, remain poorly understood. This review will focus on the proteins of the Red recombination system, with particular attention to structural and mechanistic aspects, and how the lessons learned can be applied to eukaryotic systems.
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DOI：
10.1073/pnas.121164898
发表时间：
2001-06-05
影响因子：
11.1
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