Over-expression of the RieskeFeS protein increases electron transport rates and yield in Arabidopsis

Over-expression of the RieskeFeS protein increases electron transport rates and yield in Arabidopsis
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RieskeFeS 蛋白的过度表达可增加拟南芥中的电子传输速率和产量

DOI:
10.1101/133702
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发表时间:
2017
期刊:
--
影响因子:
--
通讯作者:
Simkin A
Simkin A
中科院分区:
--
文献类型:
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作者:
Simkin A

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在这项研究中,我们产生的转基因拟南芥(拟南芥)植物过表达的Rieske FeS蛋白(PetC),细胞色素B6F(cytb6F)复合物的一个组成部分。增加这种蛋白质的水平导致cytf(PetA)和cytb6(PetB),cytb6f复合物的核心蛋白水平的伴随增加。有趣的是,在Rieske FeS过表达的植物中,光系统I(PSI)和PSII复合物中的蛋白质水平也有所增加。尽管导致这些变化的机制仍有待确定,但本文介绍的转基因植物提供了探索这一点的新工具。重要的是,Rieske FeS蛋白的过表达对拟南芥植物中PSI和PSII的量子效率、电子传递、生物量和种子产量产生了实质性和显著的影响。这些结果表明,操纵电子传递过程,以提高作物产量的潜力。
In this study, we generated transgenic Arabidopsis (Arabidopsis thaliana) plants overexpressing the Rieske FeS protein (PetC), a component of the cytochromeb6f(cytb6f) complex. Increasing the levels of this protein resulted in concomitant increases in the levels of cytf(PetA) and cytb6(PetB), core proteins of the cytb6fcomplex. Interestingly, an increase in the levels of proteins in both the photosystem I (PSI) and PSII complexes also was seen in the Rieske FeS overexpression plants. Although the mechanisms leading to these changes remain to be identified, the transgenic plants presented here provide novel tools to explore this. Importantly, overexpression of the Rieske FeS protein resulted in substantial and significant impacts on the quantum efficiency of PSI and PSII, electron transport, biomass, and seed yield in Arabidopsis plants. These results demonstrate the potential for manipulating electron transport processes to increase crop productivity.
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