Introducing “time-of-flight single particle investigator” (TOF-SPI): a tool for quantitative spICP-TOFMS data analysis

Introducing “time-of-flight single particle investigator” (TOF-SPI): a tool for quantitative spICP-TOFMS data analysis
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隆重推出“飞行时间单粒子探测器”(TOF-SPI):一种用于定量 spICP-TOFMS 数据分析的工具

DOI:
10.1039/d3ja00421j
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发表时间:
2024
影响因子:
3.4
通讯作者:
Lee, Woolin
Lee, Woolin
中科院分区:
化学2区
文献类型:
--
作者:
Gundlach-Graham, Alex;Harycki, Stasia;Szakas, Sarah E.;Taylor, Tristen L.;Karkee, Hark;Buckman, Raven L.;Mukta, Shahnaz;Hu, Rui;Lee, Woolin

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单粒子飞行时间质谱法(spICP-TOFMS)正迅速成为一种用于测量、定量和分类各种含金属纳米粒子(NPs)和亚微米粒子(μPs)的既定方法。随着研究人员开始获取更大的多样品spICP-TOFMS数据集,强大的批量分析程序是必不可少的。为了满足这一需求,我们的实验室开发并测试了一套spICP-TOFMS数据分析程序,称为“飞行时间单粒子调查员”或“TOF-SPI”。这些程序是用LabVIEW编写的,现在可以作为Windows可执行程序使用。TOF-SPI是为分析来自icpTOF(TOFWERK AG)仪器的数据而开发的,并直接与仪器生成的HDF 5文件一起工作。TOF-SPI是一个数据分析程序,它吸收了多年的spICP-TOFMS数据分析策略,以提供准确的单粒子发现,分裂事件校正,数量浓度的量化,每个粒子的元素质量数量的量化,并生成用户可读的输出报告。TOF-SPI能够对使用粒度法或在线微滴校准校准的spICP-TOFMS数据进行批量分析。在这里,我们报告了TOF-SPI的基本工作原理。
Single-particle time-of-flight mass spectrometry (spICP-TOFMS) is quickly becoming an established method for the measurement, quantification, and classification of diverse populations of metal-containing nanoparticles (NPs) and submicron particles (μPs). As researchers begin to acquire larger multi-sample spICP-TOFMS datasets, robust batch-analysis programs are essential. To meet this need, our lab has developed and tested a set of spICP-TOFMS data analysis programs called “time-of-flight single particle investigator” or “TOF-SPI.” These programs are written in LabVIEW and are now available for use as a Windows executable program. TOF-SPI is developed for the analysis of data from icpTOF (TOFWERK AG) instruments and works directly with the instrument-generated HDF5 files. TOF-SPI is a data analysis program that assimilates years of spICP-TOFMS data analysis strategies to provide accurate single-particle finding, split-event correction, quantification of number concentrations, quantification of element mass amounts per particle, and generation of user-readable output reports. TOF-SPI is capable of performing batch analyses of spICP-TOFMS data calibrated with either the particle-size method or online microdroplet calibration. Here, we report the basic operating principles of TOF-SPI.
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影响因子: 11.4
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