ROS generation and proline metabolism in calli of halophyte Nitraria tangutorum Bobr. to sodium nitroprusside treatment
ROS generation and proline metabolism in calli of halophyte Nitraria tangutorum Bobr. to sodium nitroprusside treatment
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盐生植物白刺愈伤组织中ROS的产生和脯氨酸代谢。
DOI：
10.1007/s00709-013-0527-6
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发表时间：
2013-07
期刊：
影响因子：
--
通讯作者：
Yingli Yang
中科院分区：
文献类型：
--
作者：
Xiaohui Duan &;Jing Zhang;Xiaoning Li;Yingli Yang
一氧化氮（NO）是一种胁迫因子或信号分子，参与植物的多种生理发育过程。本研究研究了不同硝普钠（SNP， NO供体）浓度对盐生白刺愈伤组织活性氧生成和脯氨酸代谢的影响。在100 μM SNP处理的愈伤组织中，过氧化氢（H2O2）含量和细胞活力显著增加，但氢自由基水平和脂质过氧化程度显著降低，超氧化物洋葱（O2(-)）含量显著增加。使用质膜（PM） NADPH氧化酶二苯基碘（DPI）化学抑制剂，我们发现未经处理和25 μM SNP处理的愈伤组织中O2（-）产生较低，而在100 μM SNP处理的愈伤组织中O2（-）水平几乎没有变化，相当于没有DPI。这表明未经处理的愈伤组织具有较高的PM NADPH氧化酶活性。不同SNP处理的愈伤组织H2O2清除酶（过氧化氢酶、过氧化物酶（POD）和抗坏血酸过氧化物酶）和H2O2形成酶（超氧化物歧化酶（SOD）、细胞壁POD和二胺氧化酶（DAO））活性显著升高，谷胱甘肽还原酶活性降低。此外，在snp处理的愈伤组织中，脯氨酸含量也有所降低。此外，不同SNP浓度刺激脯氨酸脱氢酶（PDH）和鸟氨酸δ-转氨酶（δ-氨基转移酶），抑制r-谷氨酰激酶（GK）。综上所述，我们的研究结果表明，H2O2生成的增加与SOD、细胞壁pod和DAO的刺激有关，脯氨酸含量的降低可能是唐古兰PDH活性增加和GK活性降低的结果。SNP处理下愈伤组织。
Nitric oxide (NO) is a stress factor or a signal molecule involved in various plant physiological and developmental processes. In the present study, the generation of reactive oxygen species and the metabolism of proline due to different sodium nitroprusside (SNP, an NO donor) concentrations were investigated in callus from halophyte Nitraria tangutorum Bobr. Treatment with SNP led to significant increases of hydrogen peroxide (H2O2) content and cell viability but notable reductions in hydrogen radical level and lipid peroxidation degree, and superoxide onion (O2 (-)) content also enhanced in 100 μM SNP-treated calli. Using a chemical inhibitor for plasma membrane (PM) NADPH oxidase diphenylene iodonium (DPI), we found low O2 (-) generation in untreated and 25 μM SNP-treated calli, whereas in those treated with 100 μM SNP O2 (-) level exhibited a very little alteration, comparable to the absence of DPI. These suggest a high activity of PM NADPH oxidase in untreated calli. H2O2 scavenging enzymes (catalase, peroxidase [POD] and ascorbate peroxidase) and H2O2 forming enzymes (superoxide dismutase [SOD], cell wall-POD and diamine oxidase [DAO]) stimulated significantly in calli treated with different SNP concentrations while glutathione reductase activity decreased. In addition, a reduction in proline content was observed in SNP-treated calli. Moreover, different SNP concentrations stimulated proline dehydrogenase (PDH) and ornithine δ-aminotransferase but inhibited r-glutamyl kinase (GK). In conclusion, our results suggest that the increasing H2O2 generation was associated with the stimulation of SOD, cell wall-POD and DAO, and that the reduction of proline content might be the consequence of increased PDH activity and decreased GK activity in N. tangutorum Bobr. calli under SNP treatment.
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影响因子：
6.9
作者：
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通讯作者：
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影响因子：
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作者：
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