Transcriptomic analysis of the red and green light responses in Columba livia domestica
Transcriptomic analysis of the red and green light responses in Columba livia domestica
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家鸽红光和绿光反应的转录组分析
DOI：
10.1007/s13205-018-1551-1
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发表时间：
2019-01
期刊：
影响因子：
2.8
通讯作者：
Wang Zhiyue
中科院分区：
文献类型：
--
作者：
Wang Ying;Yang Haiming;Zi Chen;Wang Zhiyue
在这项研究中，108对白色王鸽，随机分为三个隔间暴露于绿色，红光，和白色光，随后15小时的光暴露，为期6个月。从每组中选择三只雌性鸟并收集卵巢基质组织。每天记录鸽子的繁殖数据。我们使用Illumina Hiseq 2000对几个组织样本进行转录组组装，并分析了涉及卵泡发育机制的差异表达基因。繁殖数据证实，暴露于红色和绿色光改善鸽子繁殖。使用Trinity程序总共获得了平均长度为753 bp的约158，080个单基因。基因本体论，聚类的orthopathic组，和基因的京都百科全书被用来注释和分类这些unigenes。通过对单色光与白色光处理组进行成对比较，发现了大量差异表达的基因。这些基因中的一些与类固醇激素的生物合成、细胞周期和昼夜节律有关。此外，使用qRT-PCR检测随机选择的基因的相对表达水平。还确定了总共17，419个潜在的简单序列重复。我们的研究提供了对调节鸽子繁殖的潜在分子机制和基因的深入了解，以响应单色光暴露。我们的结果和数据将有助于进一步研究红色和绿色光对鸽子卵泡发育和生殖影响背后的分子机制。
In this study, 108 paired White King pigeons, randomly divided into three compartments were exposed to green light, red light, and white light followed by 15 h of light exposure, for a 6-month period. Three female birds from each group were selected and ovarian stromal tissue was collected. Pigeon reproductive data were also recorded every day. We performed transcriptome assembly on several tissue samples using Illumina Hiseq 2000 and analyzed differentially expressed genes involving follicle development mechanisms. Reproductive data confirmed that exposure to red and green lights improved pigeon reproduction. In total, approximately 158,080 unigenes with an average length of 753 bp were obtained using the Trinity program. Gene ontology, clusters of orthologous groups, and the Kyoto encyclopedia of genes were used to annotate and classify these unigenes. Large numbers of differentially expressed genes were discovered through pairwise comparisons between groups treated with monochromatic light versus white light. Some of these genes are associated with steroid hormone biosynthesis, cell cycle and circadian rhythm. Furthermore, qRT-PCR was used to detect the relative expression levels of randomly selected genes. A total of 17,419 potential simple sequence repeats were also identified. Our study provides insights into potential molecular mechanisms and genes that regulate pigeon reproduction in response to monochromatic light exposure. Our results and data will facilitate a further investigation into the molecular mechanisms behind the effects of red and green lights on follicle development and reproduction in the pigeon.
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影响因子：
5.8
作者：
Tong, Mark Y.;Cassa, Christopher A.;Kohane, Isaac S.
通讯作者：
Kohane, Isaac S.
影响因子：
--
作者：
G. Khargharia;R. Goswami;Breeding
通讯作者：
G. Khargharia;R. Goswami;Breeding
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
DOI：
10.1073/pnas.1118403109
发表时间：
2012-02-21
影响因子：
11.1
作者：
Ma, Pengpeng;Pan, Hua;Schultz, Richard M.
通讯作者：
Schultz, Richard M.
影响因子：
14.9
作者：
Kanehisa M;Araki M;Goto S;Hattori M;Hirakawa M;Itoh M;Katayama T;Kawashima S;Okuda S;Tokimatsu T;Yamanishi Y
通讯作者：
Yamanishi Y