Antagonistic effects of selenium on cadmium-induced apoptosis by restoring the mitochondrial dynamic equilibrium and energy metabolism in chicken spleens.
Antagonistic effects of selenium on cadmium-induced apoptosis by restoring the mitochondrial dynamic equilibrium and energy metabolism in chicken spleens.
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DOI：
10.18632/oncotarget.17539
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发表时间：
2017-08-08
期刊：
影响因子：
--
通讯作者：
Li S
中科院分区：
文献类型：
--
作者：
Xu Z;Jin X;Pan T;Liu T;Wan N;Li S
本研究旨在探讨镉诱导鸡脾脏细胞凋亡的机制及硒的拮抗作用。我们复制了硒-镉相互作用模型，并检测了凋亡、免疫、线粒体动力学和能量代谢相关基因的表达。结果表明，经镉处理后，细胞凋亡频率显著增加，并观察到细胞凋亡的形态学特征。促凋亡基因表达增加，抗凋亡基因表达减少。肿瘤坏死因子-α和间素-1β mRNA水平明显升高，而间素-2和干扰素-γ mRNA水平明显降低。动力蛋白相关蛋白1和线粒体分裂因子mRNA和蛋白水平显著升高，丝裂丝蛋白1、丝裂丝蛋白2和视神经萎缩蛋白1显著降低。己糖激酶1、己糖激酶2、乌头酸酶2、乳酸脱氢酶A、乳酸脱氢酶B、琥珀酸脱氢酶B、丙酮酸激酶和磷酸果糖激酶的表达也降低。添加硒显著减弱镉诱导的效应（p < 0.05）。综上所述，镉处理导致鸡脾脏线粒体动态失衡，能量代谢降低，导致细胞凋亡和免疫功能障碍，硒对镉诱导的细胞凋亡具有拮抗作用。
The aim of this study was to investigate the mechanism of cadmium-induced apoptosis in chicken spleens and the antagonistic effects of selenium. We duplicated the selenium-cadmium interaction model and examined the expression of apoptosis-, immune-, mitochondrial dynamics- and energy metabolism-related genes. The results demonstrated that after treatment with cadmium, the frequency of apoptosis was significantly increased, and the morphological characteristics of apoptosis were observed. The expression of pro-apoptotic genes was increased, and that of anti-apoptotic genes was decreased. The mRNA levels of tumor necrosis factor-α and interlenkin-1β were observably increased, but the interlenkin-2 and interferon-γ levels were markedly decreased. Furthermore, the mRNA and protein levels of dynamin-related protein 1 and mitochondrial fission factor were significantly enhanced, whereas mitofusin 1, mitofusin 2, and optic atrophy 1 were markedly decreased. The expression of hexokinase 1, hexokinase 2, aconitase 2, lactate dehydrogenase A, lactate dehydrogenase B, succinatedehydrogenase B, pyruvate kinase and phosphofructokinase were also reduced. Selenium supplements remarkably attenuated cadmium-induced effects (p < 0.05). Based on the above results, conclude that the cadmium treatment promoted a mitochondrial dynamic imbalance and reduced energy metabolism, leading to apoptosis and immune dysfunction in chicken spleens, and selenium had an antagonistic effect on Cd-induced apoptosis.
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DOI：
10.1262/jrd.2013-097
发表时间：
2014-04-24
期刊：
The Journal of reproduction and development
影响因子：
--
作者：
Eleawa SM;Alkhateeb MA;Alhashem FH;Bin-Jaliah I;Sakr HF;Elrefaey HM;Elkarib AO;Alessa RM;Haidara MA;Shatoor AS;Khalil MA
通讯作者：
Khalil MA
影响因子：
4.8
作者：
Khatri, Shikha;Yepiskoposyan, Hasmik;Plas, David R.
通讯作者：
Plas, David R.
影响因子：
2
作者：
Jameel A;Ooi KG;Jeffs NR;Galatowicz G;Lightman SL;Calder VL
通讯作者：
Calder VL
影响因子：
3.9
作者：
Guo, Ying;Zhao, Panpan;Xing, Mingwei
通讯作者：
Xing, Mingwei
影响因子：
2.5
作者：
Alaimo, Agustina;Gorojod, Roxana M.;Kotler, Monica L.
通讯作者：
Kotler, Monica L.