Aldosterone synthase gene (CYP11B2) C-344T polymorphism in Caucasians from the Berlin Salt-Sensitivity Trial (BeSST).
Aldosterone synthase gene (CYP11B2) C-344T polymorphism in Caucasians from the Berlin Salt-Sensitivity Trial (BeSST).
复制标题
来自柏林盐敏感性试验 (BeSST) 的白种人醛固酮合酶基因 (CYP11B2) C-344T 多态性。
DOI：
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发表时间：
1999
影响因子：
4.9
通讯作者：
Arya M. Sharma
中科院分区：
文献类型：
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作者：
E. Brand;U. Schorr;J. Ringel;J. Beige;A. Distler;Arya M. Sharma
客观化
醛固酮合成酶(CYP11B2)是醛固酮生物合成的关键酶。最近的研究发现，CYP11B2基因5‘侧翼区的T等位基因(C-344T)在高血压病患者中的分布频率明显高于正常血压人群，并与血浆醛固酮水平升高有关。因此，我们假设这种变异可能与饮食盐摄入引起的血压反应增加有关。
研究对象和方法
我们对在柏林盐敏感性试验(BeSST)框架内招募的1 63名血压正常的年轻男性进行了CYP11B2 C-344T多态的基因分型。受试者的特征包括高血压家族史、肾素-血管紧张素-醛固酮系统的血浆参数以及对高盐饮食(220 mmo1/天)和低盐饮食(20 mmo1/d)的血压反应
结果
-344T等位基因频率(0.45)与文献报道相似，基因型分布符合Hardy-Weinberg平衡(CC，n=55；CT，71；TT，n=37)。有高血压家族史者T等位基因频率有升高趋势(0.48：0.42)，但C-344T等位基因与血压无相关性。此外，将受试者分为盐敏感组和耐盐组，两组间的等位基因分布差异无统计学意义(Qt=0.43，Qt=0.45)。在高盐饮食(P=0.02)和低盐饮食(P=0.008)下，T等位基因携带者血浆血管紧张素原水平显著升高，而肾素活性和血浆醛固酮水平与基因型无关。
结论
我们的发现不支持这样的假设，即在血压正常的年轻受试者中，CYP11B2基因C-344T多态与盐敏感性或肾素-血管紧张素系统活性增加有关。因此，C-344T多态不太可能是血压正常的年轻高加索男性盐敏感性的遗传标志。
OBJECTIVE
Aldosterone synthase (CYP11B2) is a key enzyme in the biosynthesis of aldosterone. Recently, the T allele of a polymorphism in the 5'-flanking region of the CYP11B2 gene (C-344T) has been reported to be more frequent in hypertensives than in normotensives, and has also been associated with increased plasma aldosterone levels. We therefore hypothesized that this variant may be related to increased blood-pressure response to dietary salt intake.
SUBJECTS AND METHODS
We genotyped 1 63 young normotensive men recruited within the framework of the Berlin Salt-Sensitivity Trial (BeSST) for the CYP11B2 C-344T polymorphism. Subjects were characterized for family history of hypertension, plasma parameters of the renin-angiotensin-aldosterone system and blood-pressure response to a high (220 mmol/day) and low (20 mmol/day) salt diet
RESULTS
The frequency of the -344T allele (0.45) was similar to that reported previously and genotype distribution was in Hardy-Weinberg equilibrium (CC, n = 55; CT, n = 71; TT, n = 37). There was a trend towards a higher frequency of the T allele in subjects with a positive family history of hypertension (0.48 versus 0.42), but the C-344T genotype was not related to blood pressure under either diet Furthermore, when subjects were classified into salt-sensitive and salt-resistant groups, allelic distribution did not differ between the two groups (qT = 0.43 versus qT = 0.45). While renin activity and plasma aldosterone levels were not related to genotype, plasma angiotensinogen was significantly higher in T-allele carriers under both the high (P = 0.02) and low (P = 0.008) salt diet.
CONCLUSION
Our findings do not support the hypothesis that the C-344T polymorphism of the CYP11B2 gene is associated with salt sensitivity or increased activity of the renin-angiotensin system in young normotensive subjects. It is, therefore, unlikely that the C-344T polymorphism is a genetic marker for salt sensitivity in young normotensive Caucasian men.
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影响因子：
37.8
作者：
M. Kupari;A. Hautanen;L. Lankinen;P. Koskinen;J. Virolainen;H. Nikkila;P. White
通讯作者：
M. Kupari;A. Hautanen;L. Lankinen;P. Koskinen;J. Virolainen;H. Nikkila;P. White
影响因子：
--
作者：
C. Clyne;Y Zhang-;L. Slutsker;J. Mathis;P. White;W. Rainey
通讯作者：
C. Clyne;Y Zhang-;L. Slutsker;J. Mathis;P. White;W. Rainey
影响因子：
2.1
作者：
WHITE, PC;SLUTSKER, L
通讯作者：
SLUTSKER, L
影响因子：
2.1
作者：
M. Weinberger
通讯作者：
M. Weinberger
影响因子：
--
作者：
CURNOW, KM;TUSIELUNA, MT;WHITE, PC
通讯作者：
WHITE, PC