Characterization of spatial and temporal development of Type I and Type II hair cells in the mouse utricle using new cell-type-specific markers.
Characterization of spatial and temporal development of Type I and Type II hair cells in the mouse utricle using new cell-type-specific markers.
复制标题
DOI：
10.1242/bio.038083
复制
发表时间：
2018-11-19
期刊：
影响因子：
2.4
通讯作者：
Kelley MW
中科院分区：
文献类型：
--
作者：
McInturff S;Burns JC;Kelley MW
内耳的椭圆囊是一种介导线性加速度感知的前庭感觉结构，由两种形态和生理上不同类型的机械感觉毛细胞组成，称为 Is 型和 II 型。虽然这些细胞类型在成人椭圆囊中很容易区分，但由于缺乏可用于在成熟前识别每种细胞类型的分子标记，对它们的发育的理解受到了阻碍。因此，我们收集了三个不同年龄的单个毛细胞，并使用单细胞 RNAseq 来表征这些细胞的转录组。差异基因表达分析确定 Spp1 是 I 型毛细胞的特异性标记，Mapt 和 Anxa4 是 II 型毛细胞的特异性标记。抗体标记证实了这些标记的特异性，然后将其用于检查椭圆囊毛细胞的时间和空间发育。 I 型毛细胞以从后内侧到前外侧的梯度延伸穿过椭圆囊，而 II 型毛细胞最初在中央纹状体区域发育，然后均匀地向周边延伸。最后，通过将这些标记与遗传命运图谱相结合，我们证明超过 98% 的 I 型毛细胞在出生前发育，而超过 98% 的 II 型毛细胞在出生后发育。这些结果与之前的研究结果一致，表明 I 型毛细胞首先发育，并反驳了 II 型毛细胞代表未成熟毛细胞和 I 型毛细胞之间的过渡形式的假设。摘要：单细胞 RNAseq 用于鉴定哺乳动物椭圆囊中特定毛细胞的新标记。利用这些新标记，可以检查特定类型毛细胞的发育时间。
The utricle of the inner ear, a vestibular sensory structure that mediates perception of linear acceleration, is comprised of two morphologically and physiologically distinct types of mechanosensory hair cells, referred to as Type Is and Type IIs. While these cell types are easily discriminated in an adult utricle, understanding their development has been hampered by a lack of molecular markers that can be used to identify each cell type prior to maturity. Therefore, we collected single hair cells at three different ages and used single cell RNAseq to characterize the transcriptomes of those cells. Analysis of differential gene expression identified Spp1 as a specific marker for Type I hair cells and Mapt and Anxa4 as specific markers for Type II hair cells. Antibody labeling confirmed the specificity of these markers which were then used to examine the temporal and spatial development of utricular hair cells. While Type I hair cells develop in a gradient that extends across the utricle from posterior-medial to anterior-lateral, Type II hair cells initially develop in the central striolar region and then extend uniformly towards the periphery. Finally, by combining these markers with genetic fate mapping, we demonstrate that over 98% of all Type I hair cells develop prior to birth while over 98% of Type II hair cells develop post-natally. These results are consistent with previous findings suggesting that Type I hair cells develop first and refute the hypothesis that Type II hair cells represent a transitional form between immature and Type I hair cells. Summary: Single cell RNAseq was used to identify new markers for specific hair cell in the mammalian utricle. With these new markers, the developmental timing of specific types of hair cells was examined.
登录
查看更多内容
影响因子：
7.3
作者：
Deans, Michael R.
通讯作者：
Deans, Michael R.
影响因子：
16.6
作者：
Burns JC;Kelly MC;Hoa M;Morell RJ;Kelley MW
通讯作者：
Kelley MW
DOI：
10.1007/s10162-006-0043-x
发表时间：
2006-09-01
影响因子：
2.4
作者：
Cunningham, Lisa L.;Brandon, Carlene S.
通讯作者：
Brandon, Carlene S.
发育中大鼠外耳道 I 型毛细胞和萼传入末梢的 M 型 K+ 电流
影响因子：
5.3
作者：
Hurley, Karen M.;Gaboyard, Sophie;Eatock, Ruth Anne
通讯作者：
Eatock, Ruth Anne
影响因子：
--
作者：
Bandorowicz-Pikula, Joanna;Wos, Marcin;Pikula, Slawomir
通讯作者：
Pikula, Slawomir