A time-course study of actively stained mouse brains: Diffusion tensor imaging parameters and connectomic stability over 1 year.
A time-course study of actively stained mouse brains: Diffusion tensor imaging parameters and connectomic stability over 1 year.
复制标题
DOI：
10.1002/nbm.4611
复制
发表时间：
2022-01
影响因子：
2.9
通讯作者：
Johnson GA
中科院分区：
文献类型：
--
作者：
Xiao J;Hornburg KJ;Cofer G;Cook JJ;Pratson F;Qi Y;Johnson GA
虽然扩散张量成像（DTI）、纤维束成像和连接组学在固定组织中的应用是当今的常见做法，但在长时间内研究固定对大脑微观结构的影响的研究有限。该小鼠模型时间过程研究报告了区域脑体积和扩散标量参数的变化，例如作为脑结构稳定性测量的十二个代表性脑区域的分数各向异性。标量DTI参数和区域体积在固定后的前两周内变化很大。固定后2 - 8周的时间窗内，相同的参数是一致的，这意味着该扫描时间窗内组织稳定性的混淆是最小的。在相同的时间内分析定量连接体，延长至一年。虽然在固定后一年标量指标有一些变化，但这些变化足够小，特别是在白色物质中，以支持固定后两周到一年内的可再现连接体。这些发现描绘了一个扫描期间，脑容量，扩散标量指标和连接体是非常一致的。本测试/重新测试扩散加权（DW）MRI连接组学研究确定了固定后可以重新测量主动灌注小鼠大脑（C57 BL/6 J）并仍保持结果一致性的时间段。我们从连接组学和DW指标的混合分析中确定，在三个不同的时间段内，早期（2周前），中期（2周至8周）和晚期（8周后），早期时间段是三个时间段中最不稳定的。
While the application of diffusion tensor imaging (DTI), tractography, and connectomics to fixed tissue is a common practice today, there have been limited studies examining the effects of fixation on brain microstructure over extended periods. This mouse model time-course study reports the changes of regional brain volumes and diffusion scalar parameters, such as fractional anisotropy across twelve representative brain regions as measures of brain structural stability. The scalar DTI parameters and regional volumes were highly variable over the first two weeks after fixation. The same parameters were consistent over a two to eight-week window after fixation which means confounds from tissue stability over that scanning window are minimal. Quantitative connectomes were analyzed over the same time with extension out to one year. While there is some change in the scalar metrics at one year after fixation, these changes are sufficiently small, particularly in white matter to support reproducible connectomes over a period ranging from two weeks to one year post fixation. These findings delineate a scanning period during which brain volumes, diffusion scalar metrics and connectomes are remarkably consistent. This test/retest diffusion weighted (DW) MRI connectomics study determines the time period post-fixation that an actively perfused mouse brain (C57BL/6J) can be remeasured and still maintain consistency in result. We determined from a mix of analysis of connectomics and DW metrics that within the three distinct time periods, early (prior to 2 weeks), middle (2 weeks to 8 weeks), and late (post 8 weeks), the early time period is the most unstable of the three time periods.
登录
查看更多内容
影响因子：
3.3
作者：
Fishbein, Kenneth W.;Gluzband, Yehezkiel A.;Spencer, Richard G.
通讯作者：
Spencer, Richard G.
影响因子：
3.5
作者：
Lerch JP;Gazdzinski L;Germann J;Sled JG;Henkelman RM;Nieman BJ
通讯作者：
Nieman BJ
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
5.7
作者：
de Guzman, A. Elizabeth;Wong, Michael D.;Nieman, Brian J.
通讯作者：
Nieman, Brian J.
影响因子：
5.7
作者：
Miller, Karla L.;Stagg, Charlotte J.;Douaud, Gwenaelle;Jbabdi, Saad;Smith, Stephen M.;Behrens, Timothy E. J.;Jenkinson, Mark;Chance, Steven A.;Esiri, Margaret M.;Voets, Natalie L.;Jenkinson, Ned;Aziz, Tipu Z.;Turner, Martin R.;Johansen-Berg, Heidi;McNab, Jennifer A.
通讯作者：
McNab, Jennifer A.