Protective and antifecundity effects of Sm-p80-based DNA vaccine formulation against Schistosoma mansoni in a nonhuman primate model.
Protective and antifecundity effects of Sm-p80-based DNA vaccine formulation against Schistosoma mansoni in a nonhuman primate model.
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DOI：
10.1016/j.vaccine.2009.02.096
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发表时间：
2009-05-11
期刊：
影响因子：
5.5
通讯作者：
Siddiqui AA
中科院分区：
文献类型：
--
作者：
Ahmad G;Zhang W;Torben W;Damian RT;Wolf RF;White GL;Chavez-Suarez M;Kennedy RC;Siddiqui AA
血吸虫病是一种重要的寄生虫病，目前尚无疫苗。我们一直专注于曼氏血吸虫，Sm-p80，作为候选疫苗的功能上重要的抗原，因为其一致的免疫原性，保护潜力和抗生育作用在小鼠模型中观察到的，并在免疫逃避过程中发挥关键作用。在本研究中，我们报告说，Sm-p80为基础的DNA疫苗配方赋予38%的减少蠕虫负担的非人灵长类动物模型，狒狒（Papio anubis）。用Sm-p80-pcDNA 3免疫的动物表现出产蛋减少32%。Sm-p80 DNA在接种疫苗的动物中引起特异性免疫应答，包括IgG;其亚型IgG 1和IgG 2;和IgM。Sm-p80体外刺激免疫动物的外周血单个核细胞（PMBCs）产生的Th 1应答增强细胞因子（IL-2、IFN-γ）明显多于Th 2应答增强细胞因子（IL-4、IL-10）。在酶联免疫吸附斑点（ELISPOT）试验中，PBMC产生的INF-γ斑点形成单位明显多于IL-4。总的来说，尽管Sm-p80免疫后产生了混合型（Th 1/Th 2）体液抗体应答，但主要的保护性免疫应答是Th 1型。这些数据加强了Sm-p80作为血吸虫病优秀候选疫苗的潜力。
Schistosomiasis is an important parasitic disease for which there is no available vaccine. We have focused on a functionally important antigen of Schistosoma mansoni, Sm-p80, as a vaccine candidate because of its consistent immunogenicity, protective potential and antifecundity effect observed in murine models; and for its pivotal role in the immune evasion process. In the present study we report that a Sm-p80-based DNA vaccine formulation confers 38% reduction in worm burden in a nonhuman primate model, the baboon (Papio anubis). Animals immunized with Sm-p80-pcDNA3 exhibited a decrease in egg production by 32%. Sm-p80 DNA elicited specific immune responses that include IgG; its subtypes IgG1 and IgG2; and IgM in vaccinated animals. Peripheral blood mononuclear cells (PMBCs) from immunized animals when stimulated in vitro with Sm-p80 produced appreciably more Th1 response enhancing cytokines (IL-2, IFN-γ) than Th2 response enhancing cytokines (IL-4, IL-10). PBMCs produced appreciably more spot forming units for INF-γ than for IL-4 in enzyme-linked immunosorbent spot (ELISPOT) assays. Overall it appears that even though a mixed (Th1/Th2) type of humoral antibody response was generated following immunization with Sm-p80; the dominant protective immune response is Th1 type. These data reinforce the potential of Sm-p80 as an excellent vaccine candidate for schistosomiasis.
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影响因子：
5.5
作者：
HotaMitchell, S;Siddiqui, AA;Podesta, RB
通讯作者：
Podesta, RB
DOI：
10.1590/s0074-02762006000900061
发表时间：
2006-10-01
期刊：
Memórias do Instituto Oswaldo Cruz
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作者：
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影响因子：
5.5
作者：
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通讯作者：
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DOI：
10.1016/0925-4439(93)90087-h
发表时间：
1993-03-24
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
SIDDIQUI, AA;ZHOU, Y;CLARKE, MW
通讯作者：
CLARKE, MW