Ustilago maydis natural antisense transcript expression alters mRNA stability and pathogenesis.
Ustilago maydis natural antisense transcript expression alters mRNA stability and pathogenesis.
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DOI：
10.1111/mmi.12254
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发表时间：
2013-07
影响因子：
3.6
通讯作者：
Saville BJ
中科院分区：
文献类型：
--
作者：
Donaldson ME;Saville BJ
玉米黑粉菌感染玉米后产生厚壁二倍体冬孢子，冬孢子是玉米黑粉菌的传播媒介。该模型的生物营养型担子菌真菌的转录组分析确定了与247个开放阅读框互补的天然反义转录本（NAT）。联合对玉米NAT cDNA进行全序列测定和注释。链特异性RT-PCR屏幕确认表达，并确定优先在冬孢子中表达的NAT。有针对性的屏幕显示，四个美国。在相关真菌中保守的玉米NAT。NAT在单倍体细胞中的表达（它们不是天然存在的）导致一些互补mRNA的稳态水平增加。一个NAT，如um 02151，在单倍体细胞中的表达导致在互补mRNA水平的两倍增加，正义-反义双链RNA的形成，和不变的Um 02151蛋白水平。这导致了NAT功能的模型在存储冬孢子mRNA的维持和表达。在通过缺失调控区测试该模型时，确定NAT表达的改变导致穗轴和幼苗感染的发病率降低。这种注释和功能分析支持U的多种角色。NATs在调控基因表达和影响发病中的作用。
Ustilago maydis infection of Zea mays leads to the production of thick-walled diploid teliospores that are the dispersal agent for this pathogen. Transcriptome analyses of this model biotrophic basidiomycete fungus identified natural antisense transcripts (NATs) complementary to 247 open reading frames. The U. maydis NAT cDNAs were fully sequenced and annotated. Strand-specific RT-PCR screens confirmed expression and identified NATs preferentially expressed in the teliospore. Targeted screens revealed four U. maydis NATs that are conserved in a related fungus. Expression of NATs in haploid cells, where they are not naturally occurring, resulted in increased steady-state levels of some complementary mRNAs. The expression of one NAT, as-um02151, in haploid cells resulted in a twofold increase in complementary mRNA levels, the formation of sense–antisense double-stranded RNAs, and unchanged Um02151 protein levels. This led to a model for NAT function in the maintenance and expression of stored teliospore mRNAs. In testing this model by deletion of the regulatory region, it was determined that alteration in NAT expression resulted in decreased pathogenesis in both cob and seedling infections. This annotation and functional analysis supports multiple roles for U. maydis NATs in controlling gene expression and influencing pathogenesis.
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影响因子：
4.8
作者：
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通讯作者：
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影响因子：
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影响因子：
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