A Detoxification-Free Process for Enhanced Ethanol Production From Corn Fiber Under Semi-Simultaneous Saccharification and Fermentation.
A Detoxification-Free Process for Enhanced Ethanol Production From Corn Fiber Under Semi-Simultaneous Saccharification and Fermentation.
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半同步糖化发酵下玉米纤维提高乙醇产量的无毒工艺
DOI：
10.3389/fmicb.2022.861918
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发表时间：
2022
影响因子：
5.2
通讯作者：
中科院分区：
文献类型：
--
作者：
玉米纤维是玉米加工业的副产品，因其碳水化合物含量丰富（主要是残余淀粉、纤维素和半纤维素）、储量丰富、易于收集、几乎没有运输成本，是纤维素乙醇的有吸引力的原料。然而，玉米纤维尤其是半纤维素结构和成分复杂，难以通过酶解有效水解成可发酵糖。本研究开发了一种简单易行的工业化工艺，无需进行脱毒处理，即可以玉米纤维为原料高产乙醇。在Box-Behnken设计优化的条件下（0.5% H2SO4，105℃，43 min）对玉米纤维进行稀酸预处理，可回收81.8%的总糖，包括葡萄糖、木糖和阿拉伯糖，然后将混合物（固体和水解物）直接用于半同步糖化发酵，无需解毒，乙醇得率达到 理论收率约81%。
Corn fiber, a by-product from the corn-processing industry, is an attractive feedstock for cellulosic ethanol because of its rich carbohydrate content (mainly residual starch, cellulose, and hemicellulose), abundant reserves, easy collection, and almost no transportation cost. However, the complex structure and components of corn fiber, especially hemicellulose, make it difficult to be effectively hydrolyzed into fermentable sugars through enzymatic hydrolysis. This study developed a simple and easy industrialized process without detoxification treatment for high-yield ethanol produced from corn fiber. Corn fiber was pretreated by dilute acid under the conditions optimized by Box-Behnken design (0.5% H2SO4 at 105°C for 43 min), and 81.8% of total sugars, including glucose, xylose, and arabinose, could be recovered, then the mixture (solid and hydrolysates) was directly used for semi-simultaneous saccharification and fermentation without detoxification, and ethanol yield reached about 81% of the theoretical yield.
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