Separable bilayer microfiltration device for viable label-free enrichment of circulating tumour cells.
Separable bilayer microfiltration device for viable label-free enrichment of circulating tumour cells.
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DOI：
10.1038/srep07392
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发表时间：
2014-12-09
影响因子：
4.6
通讯作者：
Zheng SY
中科院分区：
文献类型：
--
作者：
Zhou MD;Hao S;Williams AJ;Harouaka RA;Schrand B;Rawal S;Ao Z;Brenneman R;Gilboa E;Lu B;Wang S;Zhu J;Datar R;Cote R;Tai YC;Zheng SY
The analysis of circulating tumour cells (CTCs) in cancer patients could provide important information for therapeutic management. Enrichment of viable CTCs could permit performance of functional analyses on CTCs to broaden understanding of metastatic disease. However, this has not been widely accomplished. Addressing this challenge, we present a separable bilayer (SB) microfilter for viable size-based CTC capture. Unlike other single-layer CTC microfilters, the precise gap between the two layers and the architecture of pore alignment result in drastic reduction in mechanical stress on CTCs, capturing them viably. Using multiple cancer cell lines spiked in healthy donor blood, the SB microfilter demonstrated high capture efficiency (78–83%), high retention of cell viability (71–74%), high tumour cell enrichment against leukocytes (1.7–2 × 103), and widespread ability to establish cultures post-capture (100% of cell lines tested). In a metastatic mouse model, SB microfilters successfully enriched viable mouse CTCs from 0.4–0.6 mL whole mouse blood samples and established in vitro cultures for further genetic and functional analysis. Our preliminary studies reflect the efficacy of the SB microfilter device to efficiently and reliably enrich viable CTCs in animal model studies, constituting an exciting technology for new insights in cancer research.
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影响因子：
158.5
作者：
Cristofanilli, M;Budd, GT;Hayes, DF
通讯作者：
Hayes, DF
DOI：
10.1177/2211068213494391
发表时间：
2013-12
期刊：
Journal of laboratory automation
影响因子：
--
作者：
Harouaka RA;Nisic M;Zheng SY
通讯作者：
Zheng SY
影响因子：
9.3
作者：
Harouaka, Ramdane A.;Zhou, Ming-Da;Zheng, Si-Yang
通讯作者：
Zheng, Si-Yang
DOI：
10.1158/1078-0432.ccr-10-1105
发表时间：
2010-10-15
期刊：
Clinical cancer research : an official journal of the American Association for Cancer Research
影响因子：
--
作者：
Lin HK;Zheng S;Williams AJ;Balic M;Groshen S;Scher HI;Fleisher M;Stadler W;Datar RH;Tai YC;Cote RJ
通讯作者：
Cote RJ
影响因子：
17.1
作者：
Ozkumur E;Shah AM;Ciciliano JC;Emmink BL;Miyamoto DT;Brachtel E;Yu M;Chen PI;Morgan B;Trautwein J;Kimura A;Sengupta S;Stott SL;Karabacak NM;Barber TA;Walsh JR;Smith K;Spuhler PS;Sullivan JP;Lee RJ;Ting DT;Luo X;Shaw AT;Bardia A;Sequist LV;Louis DN;Maheswaran S;Kapur R;Haber DA;Toner M
通讯作者：
Toner M