Circulating tumor cell enrichment based on physical properties.
Circulating tumor cell enrichment based on physical properties.
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DOI：
10.1177/2211068213494391
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发表时间：
2013-12
期刊：
影响因子：
--
通讯作者：
Zheng SY
中科院分区：
文献类型：
--
作者：
Harouaka RA;Nisic M;Zheng SY
恶性循环肿瘤细胞（CTC）的转移性播散和扩散占癌症相关死亡的90%以上。CTC从原发性肿瘤分离，穿过循环系统，然后在远处器官中侵入和增殖。已经建立了从血液中检测CTC用于预后监测，并且可以预测患者结果。对CTC的分析可以实现癌症的早期检测和筛查，并以微创方式提供对肿瘤组织的诊断途径。分析CTC的根本挑战是它们在血液中的浓度极低，大约为十亿分之一。已经提出了各种技术来分离CTC用于富集。在这里，我们专注于不受特异性捕获抗体限制的抗原非依赖性方法。综述了CTC的固有物理性质，包括细胞大小、变形能力和电学性质，并总结了开发利用CTC从血液中富集的技术。物理富集技术特别令人感兴趣，因为它们具有提高产量的潜力，并且能够分析可能无法以其他方式获得的罕见CTC表型。
The metastatic dissemination and spread of malignant circulating tumor cells (CTCs) accounts for over 90% of cancer related deaths. CTCs detach from a primary tumor, travel through the circulatory system, then invade and proliferate in distant organs. The detection of CTCs from blood has been established for prognostic monitoring and is predictive of patient outcome. Analysis of CTCs could enable the means for early detection and screening in cancer, as well as provide diagnostic access to tumor tissues in a minimally invasive way. The fundamental challenge with analyzing CTCs is the fact that they occur at extremely low concentrations in blood, on the order of one out of a billion cells. Various technologies have been proposed to isolate CTCs for enrichment. Here we focus on antigen-independent approaches that are not limited by specific capture antibodies. Intrinsic physical properties of CTCs including cell size, deformability, and electrical properties are reviewed, and technologies developed to exploit them for enrichment from blood are summarized. Physical enrichment technologies are of particular interest as they have the potential to increase yield, and enable the analysis of rare CTC phenotypes that may not be otherwise obtained.
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DOI：
10.1002/pros.22625
发表时间：
2013-06
期刊：
The Prostate
影响因子：
--
作者：
Chen CL;Mahalingam D;Osmulski P;Jadhav RR;Wang CM;Leach RJ;Chang TC;Weitman SD;Kumar AP;Sun L;Gaczynska ME;Thompson IM;Huang TH
通讯作者：
Huang TH
影响因子：
3.7
作者：
Cheung, K;Gawad, S;Renaud, P
通讯作者：
Renaud, P
DOI：
10.1073/pnas.92.3.860
发表时间：
1995-01-31
影响因子：
11.1
作者：
BECKER, FF;WANG, XB;GASCOYNE, PRC
通讯作者：
GASCOYNE, PRC
影响因子：
21.3
作者：
Batlle, E;Sancho, E;de Herreros, AG
通讯作者：
de Herreros, AG
影响因子：
3.4
作者：
Balic, M;Dandachi, N;Bauernhofer, T
通讯作者：
Bauernhofer, T