Antifungal and anti-inflammatory effect of punicalagin on murine Aspergillus fumigatus keratitis
Antifungal and anti-inflammatory effect of punicalagin on murine Aspergillus fumigatus keratitis
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安石榴苷对小鼠烟曲霉角膜炎的抗真菌和抗炎作用
DOI：
10.1080/02713683.2021.2008982
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发表时间：
2021-11
影响因子：
2
通讯作者：
Guiqiu Zhao
中科院分区：
文献类型：
--
作者：
Hao Lin;Qian Wang;Yawen Niu;Lingwen Gu;Li-Ting Hu;Cui Li;Guiqiu Zhao
本研究旨在探讨槟榔苷对小鼠真菌性角膜炎的抗炎和抗真菌作用。我们采用体外和体内实验方法来评价槟榔苷的抗炎和抗真菌作用。体外进行时间杀伤和菌丝染色。在体内，建立小鼠真菌性角膜炎并用PBS或PUN治疗。分别于感染后第1、3、5天进行临床评分。RT-PCR和Western blot检测各组炎症因子mRNA和蛋白水平，流式细胞术和免疫荧光检测各组巨噬细胞数量和位置。同时，用真菌平板计数来评估抗真菌效果。DCFH-DA荧光探针检测ROS水平。在体外，双关糖对烟曲霉有一定的抑制作用。MIC90值为250 μg/ml，显著降低了a.f生物膜的形成（p < 0.001）。在体内实验中，与PBS治疗组相比，经punicalagin治疗的小鼠真菌性角膜炎模型病变少，临床评分低（p < 0.05），巨噬细胞浸润减少（p < 0.001），真菌负荷降低（p < 0.001）。punicalagin还降低了促炎因子mRNA表达和蛋白水平。在细胞水平上，PUN显著降低了RAW264.7中由菌丝刺激引起的炎症因子mRNA表达和ROS产生（p < 0.001）。结果表明，黄芩苷对小鼠真菌性角膜炎有较好的治疗作用。其抗炎作用机制是综合的，包括抗真菌、抑制促炎因子和巨噬细胞、抗氧化等。
This study aimed to investigate the anti-inflammatory effect and antifungal effect of punicalagin in murine fungal keratitis.We used in vitro and in vivo protocols to assess the anti-inflammatory effect and antifungal effect of punicalagin. In vitro, time kill and mycelial stain were done. In vivo, murine fungal keratitis was established and treated with PBS or PUN. Clinical scores were taken on days 1, 3, and 5 post infection. The mRNA and protein levels of inflammatory factors were detected by RT-PCR and Western blot, and the number and location of macrophages were analyzed by flow cytometry and immunofluorescence. Also, fungal plate counting was used to assess the antifungal effect. The DCFH-DA fluorescence probe detected the ROS level.In vitro, PUN showed activity against A.fumigatus. (A.F.), with MIC90 values of 250 μg/ml, and significantly reduced A.F. biofilm formation (p < .001). In vivo, the mouse fungal keratitis model after punicalagin treatment exhibited less disease, lower clinical scores (p < .05), lower reduced macrophage infiltrate (p < .001), and fungal load (p < .001) than those treated with PBS. Treatment with punicalagin also reduced the mRNA expression and protein level of pro-inflammatory factors. At the cellular level, PUN significantly reduced the mRNA expression of inflammatory factors and ROS production caused by the stimulation of mycelia in RAW264.7 (p < .001).The results show that punicalagin is beneficial in the treatment of murine fungal keratitis. The mechanism of its anti-inflammatory effect was synthetical, including antifungal activity, an inhibitory effect of proinflammatory factor and macrophages, and anti-oxidation.
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影响因子：
30.5
作者：
通讯作者：
--
影响因子：
4.6
作者：
Xu X;Li H;Hou X;Li D;He S;Wan C;Yin P;Liu M;Liu F;Xu J
通讯作者：
Xu J
Punicalagin 是一种 PTP1B 抑制剂，通过上调小鼠巨噬细胞中的 HO-1 诱导 M2c 表型极化
影响因子：
7.4
作者：
Xu Xiaolong;Guo Yuhong;Zhao Jingxia;He Shasha;Wang Yan;Lin Yan;Wang Ning;Liu Qingquan
通讯作者：
Liu Qingquan
DOI：
10.1056/nejmoa0802629
发表时间：
2008-10-23
期刊：
The New England journal of medicine
影响因子：
--
作者：
Bochud PY;Chien JW;Marr KA;Leisenring WM;Upton A;Janer M;Rodrigues SD;Li S;Hansen JA;Zhao LP;Aderem A;Boeckh M
通讯作者：
Boeckh M
影响因子：
6.7
作者：
Levitz SM
通讯作者：
Levitz SM