Adsorption of Myoglobin and Corona Formation on Silica Nanoparticles.
Adsorption of Myoglobin and Corona Formation on Silica Nanoparticles.
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DOI：
10.1021/acs.langmuir.0c01613
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发表时间：
2020-12-01
期刊：
影响因子：
--
通讯作者：
Bharti B
中科院分区：
文献类型：
--
作者：
Lee JG;Lannigan K;Shelton WA;Meissner J;Bharti B
蛋白质从水介质中的吸附导致在纳米颗粒上形成蛋白质冠。蛋白质冠的形成受蛋白质-颗粒和蛋白质-蛋白质相互作用的复杂相互作用控制，例如静电、货车范德华、疏水、氢键和溶剂化。影响这些相互作用的实验参数，从而管理纳米颗粒上的蛋白质冠形成，目前知之甚少。这种缺乏理解是由于蛋白质分子的表面电荷分布和各向异性形状的复杂性。在这里，我们研究了pH值和盐度对肌红蛋白在二氧化硅纳米颗粒上形成的电晕特性的影响。我们实验测量和理论模型的吸附等温线结合到二氧化硅纳米粒子的肌红蛋白。通过结合吸附研究与肌红蛋白的表面静电映射，我们表明，单层硬电晕形成在低盐度的分散体，转化成一个多层的硬+软电晕后，加入盐。我们将所观察到的蛋白质吸附行为随pH值和盐度的增加而发生的变化归因于静电相互作用和表面电荷调节效应的变化。这项研究提供了对纳米颗粒上蛋白质吸附和电晕形成机制的见解，这将指导未来优化纳米颗粒设计以获得最大功能效益和最小毒性的研究。
The adsorption of proteins from aqueous medium leads to the formation of protein corona on nanoparticles. The formation of protein corona is governed by a complex interplay of protein–particle and protein–protein interactions, such as electrostatics, van der Waals, hydrophobic, hydrogen bonding, and solvation. The experimental parameters influencing these interactions, and thus governing the protein corona formation on nanoparticles, are currently poorly understood. This lack of understanding is due to the complexity in the surface charge distribution and anisotropic shape of the protein molecules. Here, we investigate the effect of pH and salinity on the characteristics of corona formed by myoglobin on silica nanoparticles. We experimentally measure and theoretically model the adsorption isotherms of myoglobin binding to silica nanoparticles. By combining adsorption studies with surface electrostatic mapping of myoglobin, we demonstrate that a monolayered hard corona is formed in low salinity dispersions, which transforms into a multilayered hard + soft corona upon the addition of salt. We attribute the observed changes in protein adsorption behavior with increasing pH and salinity to the change in electrostatic interactions and surface charge regulation effects. This study provides insights into the mechanism of protein adsorption and corona formation on nanoparticles, which would guide future studies on optimizing nanoparticle design for maximum functional benefits and minimum toxicity.
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影响因子：
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作者：
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发表时间：
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影响因子：
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