Aldehyde Dehydrogenase, a Marker of Normal and Malignant Stem Cells, Typifies Mesenchymal Progenitors in Perivascular Niches.
Aldehyde Dehydrogenase, a Marker of Normal and Malignant Stem Cells, Typifies Mesenchymal Progenitors in Perivascular Niches.
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DOI：
10.1093/stcltm/szad024
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发表时间：
2023-07-14
影响因子：
6
通讯作者：
中科院分区：
文献类型：
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先天性间充质干细胞表现出多系分化和组织（再生）生殖或致病特性，居住在血管周围的壁龛。这些祖细胞的亚群致力于骨、脂肪或纤维形成，表明血管壁中存在发育组织。我们在此评估了醛脱氢酶的活性，醛脱氢酶是催化醛氧化成羧酸的酶家族，是正常和恶性干细胞的生物标志物，在人脂肪组织血管周围区域。在图尼卡外膜中发现ALDH低至ALDH高CD 34+细胞的进展。间充质干细胞的潜力仅限于ALDHHigh细胞，如通过流式细胞术用荧光ALDH底物分选的细胞的体外增殖和多谱系分化所评估的。RNA测序证实并验证了ALDHHigh细胞具有祖细胞表型，并提供了该组分中主要同种型为ALDH1A1的证据，这通过免疫组织化学证实。这表明，ALDH活性，标志着造血祖细胞和干细胞在不同的恶性肿瘤，也代表了天然的，血管驻留间充质干细胞。包裹动脉和静脉的图尼卡外膜含有显示高ALDH活性的祖细胞，其在纯化和培养后转化为间充质干细胞。
Innate mesenchymal stem cells exhibiting multilineage differentiation and tissue (re)generative—or pathogenic—properties reside in perivascular niches. Subsets of these progenitors are committed to either osteo-, adipo-, or fibrogenesis, suggesting the existence of a developmental organization in blood vessel walls. We evaluated herein the activity of aldehyde dehydrogenase, a family of enzymes catalyzing the oxidation of aldehydes into carboxylic acids and a reported biomarker of normal and malignant stem cells, within human adipose tissue perivascular areas. A progression of ALDHLow to ALDHHigh CD34+ cells was identified in the tunica adventitia. Mesenchymal stem cell potential was confined to ALDHHigh cells, as assessed by proliferation and multilineage differentiation in vitro of cells sorted by flow cytometry with a fluorescent ALDH substrate. RNA sequencing confirmed and validated that ALDHHigh cells have a progenitor cell phenotype and provided evidence that the main isoform in this fraction is ALDH1A1, which was confirmed by immunohistochemistry. This demonstrates that ALDH activity, which marks hematopoietic progenitors and stem cells in diverse malignant tumors, also typifies native, blood vessel resident mesenchymal stem cells. The tunica adventitia, which ensheathes arteries and veins, contains progenitor cells displaying high-ALDH activity that upon purification and culture turn into mesenchymal stem cells.
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影响因子：
--
作者：
Luo, Yuchun;Nguyen, Nicholas;Fujita, Mayumi
通讯作者：
Fujita, Mayumi
影响因子：
5.7
作者：
Hida K;Maishi N;Akiyama K;Ohmura-Kakutani H;Torii C;Ohga N;Osawa T;Kikuchi H;Morimoto H;Morimoto M;Shindoh M;Shinohara N;Hida Y
通讯作者：
Hida Y
影响因子：
8.4
作者：
Choi, Seung Ah;Lee, Ji Yeoun;Kim, Seung-Ki
通讯作者：
Kim, Seung-Ki
DOI：
10.1186/s13046-018-0975-0
发表时间：
2018-12-12
影响因子：
11.3
作者：
Ciccone, Valerio;Terzuoli, Erika;Ziche, Marina
通讯作者：
Ziche, Marina
影响因子：
3.7
作者：
Hu, Zhongchang;Wu, Jian;Zhao, Yumin
通讯作者：
Zhao, Yumin