A Polycomb complex remains bound through DNA replication in the absence of other eukaryotic proteins.

A Polycomb complex remains bound through DNA replication in the absence of other eukaryotic proteins.
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DOI:
10.1038/srep00661
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发表时间:
2012
期刊:
影响因子:
4.6
通讯作者:
Francis, Nicole J.
Francis, Nicole J.
中科院分区:
综合性期刊3区
文献类型:
--
作者:
Lengsfeld, Bettina M.;Berry, Kayla N.;Ghosh, Sharmistha;Takahashi, Masateru;Francis, Nicole J.

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通过DNA复制的染色质状态的传播是表观遗传调控的核心,并且可以涉及通过与复制叉组分的相互作用将染色质蛋白募集到复制染色质。在这里,我们显示使用一个完全重建的T7噬菌体系统,真核蛋白质是不需要拴在DNA复制过程中的Polycomb复合物PRC1模板。相反,PRC1结合的DNA可以承受简单复制叉的通过。
Propagation of chromatin states through DNA replication is central to epigenetic regulation and can involve recruitment of chromatin proteins to replicating chromatin through interactions with replication fork components. Here we show using a fully reconstituted T7 bacteriophage system that eukaryotic proteins are not required to tether the Polycomb complex PRC1 to templates during DNA replication. Instead, DNA binding by PRC1 can withstand passage of a simple replication fork.
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