The apicoplast genome of Leucocytozoon caulleryi, a pathogenic apicomplexan parasite of the chicken.
The apicoplast genome of Leucocytozoon caulleryi, a pathogenic apicomplexan parasite of the chicken.
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DOI：
10.1007/s00436-013-3712-9
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发表时间：
2014-03
影响因子：
2
通讯作者：
Yukawa M
中科院分区：
文献类型：
--
作者：
Imura T;Sato S;Sato Y;Sakamoto D;Isobe T;Murata K;Holder AA;Yukawa M
卡氏住白细胞虫是一种高致病性的鸡血孢子虫。虽然这种寄生虫与兽医学非常相关，但对其基因组特征的了解有限。为了获得信息适用于开发新的控制方法的寄生虫，我们分析了顶质体基因组的L。caulleryi。该细胞器DNA为85.1% A + T，长度为34,779 bp，编码的基因与恶性疟原虫质体基因组基本相同，包括16个tRNA和30个蛋白质编码基因，除一个开放阅读框外，ORF 91在L. caulleryi。与恶性疟原虫一样，L. calleryi顶质体DNA含有两套独特的反向重复序列（IR），每一套5，253 bp，并编码指定一个大的和一个小的rRNA亚基和九个tRNA的基因，但没有蛋白质，并被一个独特的13 bp序列分开。对几种血孢子虫顶质体DNA序列的研究已经确定了相应的IR区域;然而，这些研究都没有研究完整的序列，即使是研究充分的物种，如恶性疟原虫。系统发育研究采用基于开放阅读框架的串联氨基酸序列证实了L.卡氏疟原虫属（Plasmodium spp.）在本研究中，我们确定了整个L。calleryi顶质体基因组，包括连接两个IR单位的区域。这是第一次报告的完整核苷酸序列的血孢子虫顶质体DNA与典型的IR。
Leucocytozoon caulleryi, a haemosporidian parasite of the chicken (Gallus gallus domesticus), can be highly pathogenic and often fatal. Although this parasite is extremely relevant to veterinary science, knowledge of its genomic features is limited. To gain information applicable to developing novel control methods for the parasite, we analyzed the apicoplast genome of L. caulleryi. This extranuclear organellar DNA of 85.1 % A + T and a unit of 34,779 bp was found to encode almost the same set of genes as the plastid genome of Plasmodium falciparum, including 16 tRNA and 30 protein coding genes, and except for one open reading frame, ORF91 absent in L. caulleryi. As in P. falciparum, the L. caulleryi apicoplast DNA contains two sets of a unique inverted repeat (IR), each one 5,253 bp and encoding genes specifying one large and one small rRNA subunit and nine tRNAs but no protein, and separated by a unique 13 bp sequence. Studies of several haemosporidian apicoplast DNA sequences have identified a corresponding IR region; however, none of these studies has looked at the complete sequence, even for well-studied species such as P. falciparum. Phylogenetic studies using a concatenated amino acid sequence based on the open reading frames confirmed the close relationship between L. caulleryi and Plasmodium spp. In this study, we determined the nucleotide sequence of the entire L. caulleryi apicoplast genome, including the region connecting the two IR units. This is the first report of the complete nucleotide sequence of a haemosporidian apicoplast DNA with a canonical IR.
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影响因子：
2
作者：
Oborník, M;Jirku, M;Lukes, J
通讯作者：
Lukes, J
影响因子：
2
作者：
Omori, Sumie;Sato, Yukita;Murata, Koichi
通讯作者：
Murata, Koichi
影响因子：
4
作者：
MORII, T;SHIIHARA, T;HOJI, K
通讯作者：
HOJI, K
影响因子：
5.8
作者：
Larkin, M. A.;Blackshields, G.;Higgins, D. G.
通讯作者：
Higgins, D. G.
影响因子：
5.6
作者：
Wilson, RJM;Denny, PW;Williamson, DH
通讯作者：
Williamson, DH