Acidification and sodium entry in frog skin epithelium.

Acidification and sodium entry in frog skin epithelium.
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青蛙皮肤上皮的酸化和钠进入。

DOI:
10.1016/0005-2736(81)90293-5
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发表时间:
1981
期刊:
Biochimica et biophysica acta
影响因子:
--
通讯作者:
Benos,DJ
Benos,DJ
中科院分区:
--
文献类型:
--
作者:
Benos,DJ

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研究了分离青蛙皮肤上皮(蛙蛙、临时蛙和caudiververa caudiververa)体外培养基的酸化及其与Na+摄取的关系。在短路或开路条件下,采用pH-stat技术测定酸化程度。本研究的结果表明:(a)这些物种的离体青蛙皮肤的酸化不直接与Na+或阴离子运输耦合;(b)利尿药物阿米洛利可以抑制酸化,但仅在高外部Na+浓度下;(c)这些蛙类皮肤的酸化速率部分受CO2代谢产物的控制;(d)在全动物研究中观察到的净Na+吸收和净酸化之间的正相关不能在体外皮肤制备中复制,即使青蛙第一次受到盐耗尽的长期应激,这是一种与体内实验中使用的生理状态相当的生理状态。
Acidification of the external medium by isolated frog skin epithelium (Rana catesbeiana, Rana temporaria, andCaudiververa caudiververa) and its relationship to Na+uptake was studied. Acidification was measured by the pH-stat technique under short-circuit or open-circuit conditions. The results of this study demonstrate that (a) acidification by these species of in vitro frog skins is not directly coupled to Na+or anion transport; (b) acidification can be inhibited by the diuretic drug amiloride, but only at high external Na+concentrations; (c) acidification rate in these species of frog skin is controlled in part by the metabolic production of CO2; and (d) the positive correlation between net Na+absorption and net acidification observed in whole animal studies could not be replicated in the in vitro skin preparation, even when the frogs were first chronically stressed by salt depletion, a physiological state comparable to that used in the in vivo experiments.
DOI: --
发表时间: 1976
期刊: Nature
影响因子: 64.8
作者:
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期刊: The Journal of General Physiology
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