Cardiomyocytes recruit monocytes upon SARS-CoV-2 infection by secreting CCL2.
Cardiomyocytes recruit monocytes upon SARS-CoV-2 infection by secreting CCL2.
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DOI：
10.1016/j.stemcr.2021.07.012
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发表时间：
2021-09-14
影响因子：
5.9
通讯作者：
Chen S
中科院分区：
文献类型：
--
作者：
Yang L;Nilsson-Payant BE;Han Y;Jaffré F;Zhu J;Wang P;Zhang T;Redmond D;Houghton S;Møller R;Hoagland D;Carrau L;Horiuchi S;Goff M;Lim JK;Bram Y;Richardson C;Chandar V;Borczuk A;Huang Y;Xiang J;Ho DD;Schwartz RE;tenOever BR;Evans T;Chen S
据报道，高达20%的新冠肺炎患者存在心脏损伤，但心肌组织病理学的原因仍不清楚。在这里，我们使用建立的体内仓鼠模型，证明在感染动物的心肌细胞中可以检测到SARS-CoV-2。此外，我们在仓鼠和新冠肺炎尸检样本中发现了受损的心肌细胞。为了探讨SARS-CoV-2感染的机制，我们发现人多能干细胞来源的心肌细胞和成人心肌细胞都可以被SARS-CoV-2高效感染，导致单核细胞趋化因子CCL2的分泌和随后的单核细胞募集。感染金黄地鼠心脏CCL2表达增加，单核细胞浸润增多。虽然感染的CMS受到损害，但我们发现巨噬细胞的存在显著减少了SARS-CoV-2感染的CMS。总之，我们的研究为SARS-CoV-2在体内感染CMS提供了直接的证据，并提示了新冠肺炎患者心脏组织中免疫细胞渗透和组织病理学的机制。在SARS-CoV-2感染的仓鼠心肌细胞中检测到SARS-CoV-2感染的人心肌细胞分泌CCL2 SARS-CoV-2感染的心肌细胞通过分泌CCL2巨噬细胞来招募单核细胞通过吞噬感染的CMS来减少SARS-CoV-2的感染在本文中，陈水兵博士及其同事报道了体内心肌细胞感染SARS-CoV-2的直接证据，并建立了体外模型来研究免疫细胞的渗透。他们认为，SARS-CoV-2感染导致CCL2的分泌，从而招募单核细胞迁移。
Heart injury has been reported in up to 20% of COVID-19 patients, yet the cause of myocardial histopathology remains unknown. Here, using an established in vivo hamster model, we demonstrate that SARS-CoV-2 can be detected in cardiomyocytes of infected animals. Furthermore, we found damaged cardiomyocytes in hamsters and COVID-19 autopsy samples. To explore the mechanism, we show that both human pluripotent stem cell-derived cardiomyocytes (hPSC-derived CMs) and adult cardiomyocytes (CMs) can be productively infected by SARS-CoV-2, leading to secretion of the monocyte chemoattractant cytokine CCL2 and subsequent monocyte recruitment. Increased CCL2 expression and monocyte infiltration was also observed in the hearts of infected hamsters. Although infected CMs suffer damage, we find that the presence of macrophages significantly reduces SARS-CoV-2-infected CMs. Overall, our study provides direct evidence that SARS-CoV-2 infects CMs in vivo and suggests a mechanism of immune cell infiltration and histopathology in heart tissues of COVID-19 patients. SARS-CoV-2 is detected in cardiomyocytes of SARS-CoV-2-infected hamsters SARS-CoV-2-infected human cardiomyocytes secrete CCL2 SARS-CoV-2-infected cardiomyocytes recruit monocytes by secreting CCL2 Macrophages decrease SARS-CoV-2 infection by engulfing infected CMs In this article, Dr. Shuibing Chen and colleagues report the direct evidence of SARS-CoV-2 infection of cardiomyocytes in vivo and establish an in vitro model to study immune cell infiltration. They suggest that SARS-CoV-2 infection leads to secretion of CCL2, which recruits monocyte migration.
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DOI：
10.3760/cma.j.cn112151-20200312-00193
发表时间：
2020-05-08
期刊：
Zhonghua bing li xue za zhi = Chinese journal of pathology
影响因子：
--
作者：
Yao, X H;Li, T Y;Bian, X W
通讯作者：
Bian, X W
影响因子：
24
作者：
Inciardi, Riccardo M.;Lupi, Laura;Metra, Marco
通讯作者：
Metra, Marco
影响因子：
16.6
作者：
Vallania F;Tam A;Lofgren S;Schaffert S;Azad TD;Bongen E;Haynes W;Alsup M;Alonso M;Davis M;Engleman E;Khatri P
通讯作者：
Khatri P
中国武汉市 138 例 2019 新型冠状病毒感染肺炎住院患者的临床特征。
影响因子：
120.7
作者：
Wang, Dawei;Hu, Bo;Peng, Zhiyong
通讯作者：
Peng, Zhiyong
影响因子：
5.9
作者：
Yiangou L;Davis RP;Mummery CL
通讯作者：
Mummery CL