Isolation, culture, and subculture of bovine pulmonary artery endothelial cells: Mechanical methods
Isolation, culture, and subculture of bovine pulmonary artery endothelial cells: Mechanical methods
复制标题
牛肺动脉内皮细胞的分离、培养和传代培养：机械方法
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
G. Maxwell
中科院分区：
文献类型：
--
作者：
U. Ryan;G. Maxwell
描述了通过用手术刀轻轻刮擦管腔表面从牛肺动脉(或任何大血管)机械分离内皮细胞的方法。从这些刮片中培养出单层内皮细胞。任何受污染的平滑肌细胞或成纤维细胞都用油脂铅笔圈住，当细胞继代培养时不会被移除。纯化和继代培养都是通过用橡胶警察刮刮来实现的。因此，在分离时以及在培养和继代培养过程中避免了使用蛋白水解酶的处理。
Methods are described for the mechanical isolation of endothelial cells from bovine pulmonary artery (or any large vessel) by gently scraping the luminal surface with a scalpel. Monolayers of endothelial cells are cultured from these scrapings. Any contaminating smooth muscle cells or fibroblasts are ringed with a grease pencil and not removed when the cells are subcultured. Both purification and subculture are effected by scraping with a rubber policeman. Treatment with proteolytic enzymes is thus avoided at isolation and during culture and subculture.
影响因子：
10.4
作者：
Ryan,US
通讯作者：
Ryan,US
影响因子：
2.6
作者：
RYAN, US;MORTARA, M;WHITAKER, C
通讯作者：
WHITAKER, C