OBV (obscure vein), a C(2)H(2) zinc finger transcription factor, positively regulates chloroplast development and bundle sheath extension formation in tomato (Solanum lycopersicum) leaf veins.
OBV (obscure vein), a C(2)H(2) zinc finger transcription factor, positively regulates chloroplast development and bundle sheath extension formation in tomato (Solanum lycopersicum) leaf veins.
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DOI：
10.1038/s41438-021-00659-z
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发表时间：
2021-11-01
影响因子：
8.7
通讯作者：
Li J
中科院分区：
文献类型：
--
作者：
Lu J;Pan C;Li X;Huang Z;Shu J;Wang X;Lu X;Pan F;Hu J;Zhang H;Su W;Zhang M;Du Y;Liu L;Guo Y;Li J
叶脉在植物的生长发育中起着重要作用，而维管束鞘被认为是提高C4植物光合效率的重要因素。番茄（Solanum lycopersicum）中的OBV突变体导致深色脉，并已被广泛用于加工番茄品种。然而，生理性能难以解释生产中的适合度。在这项研究中，我们证实，这种突变是由叶绿素含量增加和缺乏维管束鞘延伸（BSE）的静脉。利用全基因组关联分析和图位克隆，我们发现OBV编码C2 H2 L结构域类转录因子。该基因定位于细胞核内，在叶片中表现为细胞类型特异性表达。此外，我们通过产生番茄基因的CRISPR/Cas9敲除和过表达突变体来验证基因功能。RNA测序分析表明OBV参与了叶绿体发育和光合作用的调控，这为突变后叶绿素含量的变化提供了有力的支持。这些结果表明，OBV通过调控番茄叶片中叶绿体的发育来影响番茄的生长发育。本研究也为进一步阐明BSE的发生机制和了解陆生植物光合作用的进化提供了坚实的基础。
Leaf veins play an important role in plant growth and development, and the bundle sheath (BS) is believed to greatly improve the photosynthetic efficiency of C4 plants. The OBV mutation in tomato (Solanum lycopersicum) results in dark veins and has been used widely in processing tomato varieties. However, physiological performance has difficulty explaining fitness in production. In this study, we confirmed that this mutation was caused by both the increased chlorophyll content and the absence of bundle sheath extension (BSE) in the veins. Using genome-wide association analysis and map-based cloning, we revealed that OBV encoded a C2H2L domain class transcription factor. It was localized in the nucleus and presented cell type-specific gene expression in the leaf veins. Furthermore, we verified the gene function by generating CRISPR/Cas9 knockout and overexpression mutants of the tomato gene. RNA sequencing analysis revealed that OBV was involved in regulating chloroplast development and photosynthesis, which greatly supported the change in chlorophyll content by mutation. Taken together, these findings demonstrated that OBV affected the growth and development of tomato by regulating chloroplast development in leaf veins. This study also provides a solid foundation to further decipher the mechanism of BSEs and to understand the evolution of photosynthesis in land plants.
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影响因子：
5.6
作者：
Fan M;Gao S;Ren J;Yang Q;Li H;Yang C;Ye Z
通讯作者：
Ye Z
影响因子：
7.2
作者：
Fryer, MJ;Ball, L;Baker, NR
通讯作者：
Baker, NR
影响因子：
3
作者：
Barrios-Masias, Felipe H.;Chetelat, Roger T.;Jackson, Louise E.
通讯作者：
Jackson, Louise E.
影响因子：
9.4
作者：
CHOONG, MF;LUCAS, PW;TURNER, IM
通讯作者：
TURNER, IM
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF