Effects of Puerariae Radix Extract on Endotoxin Receptors and TNF-α Expression Induced by Gut-Derived Endotoxin in Chronic Alcoholic Liver Injury.
Effects of Puerariae Radix Extract on Endotoxin Receptors and TNF-α Expression Induced by Gut-Derived Endotoxin in Chronic Alcoholic Liver Injury.
复制标题
葛根提取物对慢性酒精性肝损伤中肠源性内毒素诱导的内毒素受体和 TNF-α 表达的影响。
DOI：
10.1155/2012/234987
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Hu YY
中科院分区：
文献类型：
--
作者：
Peng JH;Cui T;Sun ZL;Huang F;Chen L;Xu L;Feng Q;Hu YY
葛根是最早用于治疗酒精滥用的药用植物之一，已有一千多年的历史。然而，关于其对慢性酒精性肝损伤的影响，人们知之甚少。因此，本研究观察了葛根提取物对慢性酒精性肝损伤大鼠和巨噬细胞系的影响以及对肠源性内毒素诱导的库普弗细胞(KCs)释放肿瘤坏死因子-α(TN-α)的影响。观察RPE对肝组织的病理改变、脂质沉积以及血清丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)和肝脏γ-谷氨酰转肽酶(GGT)活性的影响。同时抑制慢性酒精摄入大鼠KCs活化及随后肝脏肿瘤坏死因子-α的表达，下调内毒素受体、脂多糖结合蛋白、CD14、TLR2和TLR4的蛋白表达。此外，体外研究表明，人参皂苷可抑制脂多糖诱导的RAW264.7细胞肿瘤坏死因子-α和内毒素受体CD14和TLR4的表达。综上所述，本研究证实了红景天对慢性酒精摄入所致的大鼠肝损伤和脂质沉积，以及体内和体外的肿瘤坏死因子-α的释放、内毒素受体的蛋白表达均有一定的减轻作用。
Kudzu (Pueraria lobata) is one of the earliest medicinal plants used to treat alcohol abuse in traditional Chinese medicine for more than a millennium. However, little is known about its effects on chronic alcoholic liver injury. Therefore, the present study observed the effects of puerariae radix extract (RPE) on chronic alcoholic liver injury as well as Kupffer cells (KCs) activation to release tumor necrosis factor alpha (TNF-α) induced by gut-derived endotoxin in rats and macrophage cell line. RPE was observed to alleviate the pathological changes and lipids deposition in liver tissues as well as the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), and hepatic gamma-glutamyl transpeptidase (GGT) activity. Meanwhile, RPE inhibited KCs activation and subsequent hepatic TNF-α expression and downregulated the protein expression of endotoxin receptors, lipopolysaccharide binding protein (LBP), CD14, Toll-like receptor (TLR) 2, and TLR4 in chronic alcohol intake rats. Furthermore, an in vitro study showed that RPE inhibited the expression of TNF-α and endotoxin receptors, CD14 and TLR4, induced by LPS in RAW264.7 cells. In summary, this study demonstrated that RPE mitigated liver damage and lipid deposition induced by chronic alcohol intake in rats, as well as TNF-α release, protein expression of endotoxin receptors in vivo or in vitro.
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DOI：
10.1111/j.1530-0277.2009.01031.x
发表时间：
2009-11
期刊：
Alcoholism, clinical and experimental research
影响因子：
--
作者：
Arolfo MP;Overstreet DH;Yao L;Fan P;Lawrence AJ;Tao G;Keung WM;Vallee BL;Olive MF;Gass JT;Rubin E;Anni H;Hodge CW;Besheer J;Zablocki J;Leung K;Blackburn BK;Lange LG;Diamond I
通讯作者：
Diamond I
影响因子：
7.1
作者：
Lin, RC;Li, TK
通讯作者：
Li, TK
影响因子：
3.6
作者：
Overstreet, DH;Kralic, JE;Lee, DY
通讯作者：
Lee, DY
影响因子：
2.8
作者：
Bykov, I;Järveläinen, H;Lindros, K
通讯作者：
Lindros, K
DOI：
10.1124/jpet.102.047852
发表时间：
2003-06-01
影响因子：
3.5
作者：
Lambert, JC;Zhou, ZX;Kang, YJ
通讯作者：
Kang, YJ