Multipotent adult hippocampal progenitor cells maintained as neurospheres favor differentiation toward glial lineages.
Multipotent adult hippocampal progenitor cells maintained as neurospheres favor differentiation toward glial lineages.
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作为神经球维持的多能成年海马祖细胞有利于向神经胶质谱系的分化。
DOI：
10.1002/biot.201400019
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发表时间：
2014
影响因子：
4.7
通讯作者：
Sakaguchi,DonaldS
中科院分区：
文献类型：
--
作者：
Oh,Jisun;Daniels,GabrielleJ;Chiou,LawrenceS;Ye,Eun-Ah;Jeong,Yong-Seob;Sakaguchi,DonaldS
成年海马祖细胞（AHPCs）通常维持为多能神经祖细胞的分散单层群体。为了更好地理解神经祖细胞之间的细胞-细胞相互作用及其对细胞特性的影响，我们从AHPC的粘附单层群体中产生了自由漂浮的细胞聚集体或神经球。体外分析的结果表明，这两个群体的AHPC在维持条件下高度增殖，但AHPC形成的神经球有利于分化沿着胶质细胞谱系，并显示出更大的迁移活性比传统培养的AHPC。为了研究来自两个群体的AHPC在体内的可塑性，我们通过眼内注射将表达绿色荧光蛋白（GFP）的AHPC移植到发育中的大鼠眼中。两种AHPC群体都能够存活并整合到发育中的宿主中枢神经系统中，但与贴壁AHPC相比，在移植有神经球AHPC的视网膜中观察到更多的GFP阳性细胞。这些结果表明，在维持神经祖细胞（NPC）的培养配置影响细胞的命运和运动在体外以及在体内。我们的发现对于根据不同的细胞间结构理解NPC的不同细胞特征以及使用谱系定向NPC开发基于细胞的治疗策略具有意义。
Adult hippocampal progenitor cells (AHPCs) are generally maintained as a dispersed monolayer population of multipotent neural progenitors. To better understand cell–cell interactions among neural progenitors and their influences on cellular characteristics, we generated free‐floating cellular aggregates, or neurospheres, from the adherent monolayer population of AHPCs. Results from in vitro analyses demonstrated that both populations of AHPCs were highly proliferative under maintenance conditions, but AHPCs formed in neurospheres favored differentiation along a glial lineage and displayed greater migrational activity than the traditionally cultured AHPCs. To study the plasticity of AHPCs from both populations in vivo, we transplanted green fluorescent protein (GFP)‐expressing AHPCs via intraocular injection into the developing rat eyes. Both AHPC populations were capable of surviving and integrating into developing host central nervous system, but considerably more GFP‐positive cells were observed in the retinas transplanted with neurosphere AHPCs, compared to adherent AHPCs. These results suggest that the culture configuration during maintenance for neural progenitor cells (NPCs) influences cell fate and motility in vitro as well as in vivo. Our findings have implication for understanding different cellular characteristics of NPCs according to distinct intercellular architectures and for developing cell‐based therapeutic strategies using lineage‐committed NPCs.
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影响因子：
13.9
作者：
Kriegstein A;Alvarez-Buylla A
通讯作者：
Alvarez-Buylla A
成体神经干细胞的免疫控制。
影响因子：
--
作者：
Gonzalez-Perez,Oscar;Quiñones-Hinojosa,Alfredo;Garcia-Verdugo,JoseManuel
通讯作者：
Garcia-Verdugo,JoseManuel
影响因子：
3.4
作者：
A. B. Wojciechowski;U. Englund;C. Lundberg;K. Wictorin;K. Warfvinge
通讯作者：
A. B. Wojciechowski;U. Englund;C. Lundberg;K. Wictorin;K. Warfvinge
影响因子：
2.9
作者：
Lu, B;Kwan, T;Young, MJ
通讯作者：
Young, MJ
影响因子：
3.1
作者：
Harper, Matthew M.;Ye, Eun-Ah;Sakaguchi, Donald S.
通讯作者：
Sakaguchi, Donald S.