Mass spectrometric assay for analysis of haptoglobin fucosylation in pancreatic cancer.
Mass spectrometric assay for analysis of haptoglobin fucosylation in pancreatic cancer.
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DOI：
10.1021/pr200102h
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发表时间：
2011-05-06
影响因子：
4.4
通讯作者：
Lubman, David M.
中科院分区：
文献类型：
--
作者：
Lin, Zhenxin;Simeone, Diane M.;Anderson, Michelle A.;Brand, Randall E.;Xie, Xiaolei;Shedden, Kerby A.;Ruffin, Mack T.;Lubman, David M.
建立了一种质谱学方法来阐明血清糖蛋白的N-糖链结构，并利用岩藻糖化的糖链作为胰腺癌的潜在标志物。用该方法测定了16例胰腺癌患者和15例良性疾病(5例正常人、5例慢性胰腺炎和5例II型糖尿病)患者血清中的结合珠蛋白。该方法仅使用10uL的血清，用单抗提取结合珠蛋白，并对所需分析的N-糖链进行定量甲基化，然后进行MALDI-QIT-TOF MS分析。鉴定了8种结合珠蛋白的N-糖链结构，其中一种双糖基化的三触角结构在胰腺癌样本中是首次报道。与良性疾病的样本相比，胰腺癌样本的核心和触角岩藻糖基化均升高。计算各期胰腺癌患者的岩藻糖化程度指数，与所分析的良性病变相比，差异有统计学意义。这项研究表明，基于结合珠蛋白岩藻糖化模式的血清分析可能是诊断胰腺癌的一种新方法。
A mass spectrometric method was developed to elucidate the N-glycan structures of serum glycoproteins and utilize fucosylated glycans as potential markers for pancreatic cancer. This assay was applied to haptoglobin in human serum where N-glycans derived from the serum of 16 pancreatic cancer patients were compared with those from 15 individuals with benign conditions (5 normals, 5 chronic pancreatitis, and 5 type II diabetes). This assay used only 10uL of serum where haptoglobin was extracted using a monoclonal antibody and quantitative permethylation was performed on desialylated N-glycans followed by MALDI-QIT-TOF MS analysis. Eight desialylated N-glycan structures of haptoglobin were identified where a bifucosylated tri-antennary structure was reported for the first time in pancreatic cancer samples. Both core and antennary fucosylation were elevated in pancreatic cancer samples compared to samples from benign conditions. Fucosylation degree indices were calculated and show a significant difference between pancreatic cancer patients of all stages and the benign conditions analyzed. This study demonstrates that a serum assay based on haptoglobin fucosylation patterns using mass spectrometric analysis may serve as a novel method for the diagnosis of pancreatic cancer.
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影响因子：
7
作者：
Kirmiz, Crystal;Li, Bensheng;Miyamoto, Suzanne
通讯作者：
Miyamoto, Suzanne
影响因子：
6.4
作者：
Okuyama, N;Ide, Y;Miyoshi, E
通讯作者：
Miyoshi, E
DOI：
10.1016/s1570-0232(02)00671-2
发表时间：
2002-12-25
影响因子：
3
作者：
Xiong, L;Regnier, FE
通讯作者：
Regnier, FE
影响因子：
5.2
作者：
Yoon, Seon-Joo;Park, Seung-Yeol;Hakomori, Sen-Itiroh
通讯作者：
Hakomori, Sen-Itiroh
影响因子：
2
作者：
Sarrats, Ariadna;Saldova, Radka;Peracaula, Rosa
通讯作者：
Peracaula, Rosa